Research Institute for Scientists Emeriti, Drew University, 36 Madison Avenue, Madison, NJ 07940, USA.
BioInvenu, 50 Williams Parkway, Unit A2, East Hanover, NJ 07936, USA.
Drug Discov Today. 2023 Aug;28(8):103641. doi: 10.1016/j.drudis.2023.103641. Epub 2023 May 24.
The activation of G-protein-coupled receptors (GPCRs) triggers a series of protein-protein interaction events that subsequently induce a chain of reactions, including alteration of receptor structures, phosphorylation, recruitment of associated proteins, protein trafficking and gene expression. Multiple GPCR signaling transduction pathways are evident - two well-studied pathways are the GPCR-mediated G-protein and β-arrestin pathways. Recently, ligand-induced interactions between GPCRs and 14-3-3 proteins have been demonstrated. This linking of GPCRs to 14-3-3 protein signal hubs opens up a whole new realm of signal transduction possibilities. 14-3-3 proteins play a key part in GPCR trafficking and signal transduction. GPCR-mediated 14-3-3 protein signaling can be harnessed for the study of GPCR function and therapeutics.
G 蛋白偶联受体 (GPCR) 的激活触发了一系列蛋白质-蛋白质相互作用事件,随后诱导了一连串的反应,包括受体结构的改变、磷酸化、相关蛋白的募集、蛋白质运输和基因表达。有多种 GPCR 信号转导途径——两种研究得比较透彻的途径是 GPCR 介导的 G 蛋白和β-arrestin 途径。最近,已经证明了配体诱导的 GPCR 与 14-3-3 蛋白之间的相互作用。这种将 GPCR 与 14-3-3 蛋白信号枢纽连接起来,为信号转导开辟了全新的可能性。14-3-3 蛋白在 GPCR 运输和信号转导中起着关键作用。GPCR 介导的 14-3-3 蛋白信号可以被利用来研究 GPCR 功能和治疗方法。