前体 mRNA 剪接及其共转录连接。
Pre-mRNA splicing and its cotranscriptional connections.
机构信息
Department of Biochemistry and Molecular Genetics, RNA Bioscience Initiative, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
Department of Biochemistry and Molecular Genetics, RNA Bioscience Initiative, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
出版信息
Trends Genet. 2023 Sep;39(9):672-685. doi: 10.1016/j.tig.2023.04.008. Epub 2023 May 24.
Transcription of eukaryotic genes by RNA polymerase II (Pol II) yields RNA precursors containing introns that must be spliced out and the flanking exons ligated together. Splicing is catalyzed by a dynamic ribonucleoprotein complex called the spliceosome. Recent evidence has shown that a large fraction of splicing occurs cotranscriptionally as the RNA chain is extruded from Pol II at speeds of up to 5 kb/minute. Splicing is more efficient when it is tethered to the transcription elongation complex, and this linkage permits functional coupling of splicing with transcription. We discuss recent progress that has uncovered a network of connections that link splicing to transcript elongation and other cotranscriptional RNA processing events.
真核基因由 RNA 聚合酶 II（Pol II）转录产生含有内含子的 RNA 前体，这些内含子必须被剪接掉，侧翼的外显子连接在一起。剪接是由一种称为剪接体的动态核糖核蛋白复合物催化的。最近的证据表明，当 RNA 链以高达 5kb/min 的速度从 Pol II 中挤出时，很大一部分剪接是共转录的。当剪接与转录延伸复合物连接时，剪接效率更高，这种连接允许剪接与转录的功能偶联。我们讨论了最近的进展，这些进展揭示了一个连接剪接与转录延伸和其他共转录 RNA 加工事件的连接网络。
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