Wu Di, Liu Jingyu, Zhou Changcheng, Ma Wenjie, Zhou Liuhua, Ge Yuzheng, Jia Ruipeng
Department of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Bioengineering (Basel). 2023 Apr 24;10(5):509. doi: 10.3390/bioengineering10050509.
Renal ischemia-reperfusion injury (IRI) is a significant cause of acute kidney injury (AKI) and usually brings severe public health consequences. Adipose-derived endothelial progenitor cell (AdEPCs) transplantation is beneficial for AKI but suffers from low delivery efficiency. This study was conducted to explore the protective effects of magnetically delivered AdEPCs on the repair of renal IRI. Two types of magnetic delivery methods, namely the endocytosis magnetization (EM) method and the immunomagnetic (IM) method were fabricated using PEG@FeO and CD133@FeO, and their cytotoxicities in AdEPCs were assessed. In the renal IRI rat model, magnetic AdEPCs were injected via the tail vein and a magnet was placed beside the injured kidney for magnetic guidance. The distribution of transplanted AdEPCs, renal function, and tubular damage were evaluated. Our results suggested that CD133@FeO had the minimum negative effects on the proliferation, apoptosis, angiogenesis, and migration of AdEPCs compared with PEG@FeO. Renal magnetic guidance could significantly enhance the transplantation efficiency and the therapeutic outcomes of AdEPCs-PEG@FeO and AdEPCs-CD133@FeO in the injured kidneys. However, under renal magnetic guidance, AdEPCs-CD133@FeO had stronger therapeutic effects than PEG@FeO after renal IRI. The immunomagnetic delivery of AdEPCs with CD133@FeO could be a promising therapeutic strategy for renal IRI.
肾缺血再灌注损伤（IRI）是急性肾损伤（AKI）的重要原因，通常会带来严重的公共卫生后果。脂肪来源的内皮祖细胞（AdEPCs）移植对AKI有益，但存在递送效率低的问题。本研究旨在探讨磁性递送的AdEPCs对肾IRI修复的保护作用。使用PEG@FeO和CD133@FeO构建了两种磁性递送方法，即内吞磁化（EM）法和免疫磁（IM）法，并评估了它们对AdEPCs的细胞毒性。在肾IRI大鼠模型中，经尾静脉注射磁性AdEPCs，并在受损肾脏旁放置磁体进行磁引导。评估移植的AdEPCs的分布、肾功能和肾小管损伤情况。我们的结果表明，与PEG@FeO相比，CD133@FeO对AdEPCs的增殖、凋亡、血管生成和迁移的负面影响最小。肾磁引导可显著提高AdEPCs-PEG@FeO和AdEPCs-CD133@FeO在受损肾脏中的移植效率和治疗效果。然而，在肾磁引导下，肾IRI后AdEPCs-CD133@FeO的治疗效果比PEG@FeO更强。用CD133@FeO对AdEPCs进行免疫磁递送可能是一种有前景的肾IRI治疗策略。
