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基于浓缩生长因子调控细胞分化的牙髓再生机制

Mechanism of Pulp Regeneration Based on Concentrated Growth Factors Regulating Cell Differentiation.

作者信息

Yu Sijing, Zheng Yi, Guo Qiang, Li Wenxu, Ye Ling, Gao Bo

机构信息

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

出版信息

Bioengineering (Basel). 2023 Apr 25;10(5):513. doi: 10.3390/bioengineering10050513.

DOI:10.3390/bioengineering10050513
PMID:37237583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10215240/
Abstract

Concentrated growth factors (CGF) is the newest generation platelet concentrate product, which has been reported to promote the proliferation and differentiation of human dental pulp cells (hDPCs). However, the effect of liquid phase of CGF (LPCGF) has not been reported. This study was aimed to evaluate the influence of LPCGF on the biological properties of hDPCs, and to explore the in vivo mechanism of dental pulp regeneration based on the hDPCs-LPCGF complex transplantation. It was found that LPCGF could promote the proliferation, migration and odontogenic differentiation of hDPCs, and 25% LPCGF induced the most mineralization nodule formation and the highest DSPP gene expression. The heterotopic transplantation of the hDPCs-LPCGF complex resulted in the formation of regenerative pulp tissue with newly formed dentin, neovascularization and nerve-like tissue. Together, these findings provide key data on the effect of LPCGF on the proliferation, migration, odontogenic/osteogenic differentiation of hDPCs, and the in vivo mechanism of hDPCs-LPCGF complex autologous transplantation in pulp regeneration therapy.

摘要

浓缩生长因子（CGF）是新一代血小板浓缩产品，据报道其可促进人牙髓细胞（hDPCs）的增殖和分化。然而，CGF液相（LPCGF）的作用尚未见报道。本研究旨在评估LPCGF对hDPCs生物学特性的影响，并基于hDPCs-LPCGF复合物移植探索牙髓再生的体内机制。研究发现，LPCGF可促进hDPCs的增殖、迁移和牙源性分化，25%的LPCGF诱导形成的矿化结节最多且DSPP基因表达最高。hDPCs-LPCGF复合物的异位移植导致形成具有新形成牙本质、新血管形成和神经样组织的再生牙髓组织。总之，这些发现提供了关于LPCGF对hDPCs增殖、迁移、牙源性/成骨分化的影响以及hDPCs-LPCGF复合物自体移植在牙髓再生治疗中的体内机制的关键数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/4721fcbd7ce9/bioengineering-10-00513-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/ff25f453b599/bioengineering-10-00513-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/a1f729c2e4d2/bioengineering-10-00513-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/7ebfa05bd831/bioengineering-10-00513-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/4721fcbd7ce9/bioengineering-10-00513-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/b59f609a9ddf/bioengineering-10-00513-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/a9dbe46ee202/bioengineering-10-00513-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/70ebbad1baa4/bioengineering-10-00513-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/e990cc685d53/bioengineering-10-00513-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/ff25f453b599/bioengineering-10-00513-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/a1f729c2e4d2/bioengineering-10-00513-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/7ebfa05bd831/bioengineering-10-00513-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0245/10215240/4721fcbd7ce9/bioengineering-10-00513-g008.jpg

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