Department of Immunopathology and Molecular Biology, Wroclaw Medical University, Bujwida 44, 50-345 Wrocław, Poland.
Int J Mol Sci. 2021 Nov 6;22(21):12018. doi: 10.3390/ijms222112018.
The therapeutic potential of the dental pulp stem (DSC) cell-derived secretome, consisting of various biomolecules, is undergoing intense research. Despite promising in vitro and in vivo studies, most DSC secretome-based therapies have not been implemented in human medicine because the paracrine effect of the bioactive factors secreted by human dental pulp stem cells (hDPSCs) and human exfoliated deciduous teeth (SHEDs) is not completely understood. In this review, we outline the current data on the hDPSC- and SHED-derived secretome as a potential candidate in the regeneration of bone, cartilage, and nerve tissue. Published reports demonstrate that the dental MSC-derived secretome/conditional medium may be effective in treating neurodegenerative diseases, neural injuries, cartilage defects, and repairing bone by regulating neuroprotective, anti-inflammatory, antiapoptotic, and angiogenic processes through secretome paracrine mechanisms. Dental MSC-secretomes, similarly to the bone marrow MSC-secretome activate molecular and cellular mechanisms, which determine the effectiveness of cell-free therapy. Many reports emphasize that dental MSC-derived secretomes have potential application in tissue-regenerating therapy due to their multidirectional paracrine effect observed in the therapy of many different injured tissues.
牙髓干细胞（DSC）细胞衍生的分泌组由各种生物分子组成，具有治疗潜力，目前正在进行深入研究。尽管体外和体内研究有希望，但由于人类牙髓干细胞（hDPSCs）和人类脱落乳牙（SHED）分泌的生物活性因子的旁分泌作用尚未完全了解，因此大多数基于 DSC 分泌组的治疗方法尚未在人类医学中实施。在这篇综述中，我们概述了 hDPSC 和 SHED 衍生的分泌组作为骨、软骨和神经组织再生的潜在候选物的最新数据。已发表的报告表明，牙间充质干细胞衍生的分泌组/条件培养基可能通过旁分泌机制通过调节神经保护、抗炎、抗细胞凋亡和血管生成过程，在治疗神经退行性疾病、神经损伤、软骨缺陷和修复骨骼方面有效。牙间充质干细胞分泌组与骨髓间充质干细胞分泌组一样，激活分子和细胞机制，这决定了无细胞治疗的有效性。许多报告强调，由于在治疗许多不同损伤组织中观察到的多向旁分泌作用，牙间充质干细胞衍生的分泌组具有组织再生治疗的潜在应用。
