Hwang Hyeon-Ji, Li Dan-Dan, Lee Jieun, Kang Min Kyung, Moon Hyung Ryong, Lee Joon-Hee
College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea.
Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea.
Antibiotics (Basel). 2023 May 5;12(5):853. doi: 10.3390/antibiotics12050853.
Two synthetic compounds, MHY1383, azo-resveratrol and MHY1387, 5-[4-hydroxy-3,5-methoxybenzy]-2-thioxodihydropyrimidine-4,6[1H,5H]-dione have been reported to have an anti-biofilm effect on at very low concentrations (1-10 pM). Here, we investigated the anti-biofilm effects of these compounds in various bacteria. We found that MHY1383 significantly inhibited , , and biofilm formation at 1 pM, 1 nM, and 10 nM, respectively. MHY1387 also inhibited the biofilm formation of , , and at 1 pM, 10 nM, and 100 pM, respectively. Both MHY1383 and MHY1387 showed medium-dependent anti-biofilm effects on at high concentrations (10 μM). We also tested the susceptibility to antibiotics by measuring the minimum inhibitory concentration (MIC) in various bacteria. When , , , , and were treated with MHY1383 or MHY1387 in combination with four different antibiotics, the MICs of carbenicillin against and were lowered more than two-fold by the combination with MHY1387. However, in all other combinations, the MIC changed within two-fold. The results of this study suggest that MHY1383 and MHY1387 are effective anti-biofilm agents and can be used at very low concentrations against biofilms formed by various types of bacteria. We also suggest that even if a substance that inhibits biofilm is used together with antibiotics, it does not necessarily have the effect of lowering the MIC of the antibiotics.
据报道，两种合成化合物，即MHY1383（偶氮白藜芦醇）和MHY1387（5-[4-羟基-3,5-甲氧基苄基]-2-硫代二氢嘧啶-4,6[1H,5H]-二酮）在极低浓度（1-10皮摩尔）下对[具体细菌名称未给出]具有抗生物膜作用。在此，我们研究了这些化合物在多种细菌中的抗生物膜作用。我们发现，MHY1383分别在1皮摩尔、1纳摩尔和10纳摩尔时显著抑制[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]的生物膜形成。MHY1387也分别在1皮摩尔、10纳摩尔和100皮摩尔时抑制[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]的生物膜形成。MHY1383和MHY1387在高浓度（10微摩尔）时对[具体细菌名称未给出]均表现出培养基依赖性抗生物膜作用。我们还通过测量多种细菌的最低抑菌浓度（MIC）来测试其对抗生素的敏感性。当[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]用MHY1383或MHY1387与四种不同抗生素联合处理时，与MHY1387联合使用时，羧苄青霉素对[具体细菌名称未给出]和[具体细菌名称未给出]的MIC降低了两倍以上。然而，在所有其他联合使用中，MIC的变化在两倍以内。本研究结果表明，MHY1383和MHY1387是有效的抗生物膜剂，可在极低浓度下用于对抗由多种细菌形成的生物膜。我们还表明，即使将抑制生物膜的物质与抗生素一起使用，也不一定具有降低抗生素MIC的作用。
