Zhang Huakun, Li Run, Wang Yaohui, Zhou Jinxu, Xu Hao, Gou Meng, Ye Jianhua, Qiu Xuemei, Wang Xiuli
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Key Laboratory of Pufferfish Breeding and Culture in Liaoning Province, Dalian Ocean University, Dalian 116023, China.
Animals (Basel). 2023 May 9;13(10):1572. doi: 10.3390/ani13101572.
has relatively small gills and gill pores, leading to a relatively low respiratory capacity and increased vulnerability to low dissolved oxygen (DO) levels compared to other fish. To investigate the responses of to acute hypoxic stress, high-throughput-sequencing-based transcriptomic analyses were conducted here to assess the responses of gills to acute hypoxic stress. Three environmental conditions were compared including normoxia (DO: 7.0 ± 0.2 mg/L), hypoxic stress (DO: 0.9 ± 0.2 mg/L), and reoxygenation (4, 8, 12, and 24 h after return to normoxia) conditions to identify differentially expressed genes (DEGs) responsive to hypoxia. A total of 992, 877, 1561, 1412, and 679 DEGs were identified in the normoxia and reoxygenation for 4, 8, 12, and 24 h groups in comparison to the hypoxia groups, respectively. The DEGs were primarily associated with oxidative stress, growth and development, and immune responses. Further functional annotation enrichment analysis of the DEGs revealed that they were primarily related to cytokine-cytokine interactions, transforming growth factor β receptor (TGF-β), cell adhesion molecules (CAMs), the vascular endothelial growth factor (VEGF) signaling pathway, and the mitogen-activated protein kinase (MAPK) signaling pathway. These results provide new insights into the physiological and biochemical mechanisms of adaptations to hypoxic stress. Furthermore, these results provide a framework for future studies into the molecular mechanisms of hypoxia tolerance and the healthy culture of and other fish.
具有相对较小的鳃和鳃孔，与其他鱼类相比，导致呼吸能力相对较低，并且对低溶解氧（DO）水平的脆弱性增加。为了研究[鱼的种类未明确]对急性缺氧应激的反应，在此进行了基于高通量测序的转录组分析，以评估[鱼的种类未明确]鳃对急性缺氧应激的反应。比较了三种环境条件，包括常氧（DO：7.0±0.2mg/L）、缺氧应激（DO：0.9±0.2mg/L）和复氧（恢复常氧后4、8、12和24小时）条件，以鉴定对缺氧有反应的差异表达基因（DEG）。与缺氧组相比，在常氧组以及复氧4、8、12和24小时组中分别鉴定出992、877、1561、1412和679个DEG。这些DEG主要与氧化应激、生长发育和免疫反应相关。对DEG的进一步功能注释富集分析表明，它们主要与细胞因子-细胞因子相互作用、转化生长因子β受体（TGF-β）、细胞粘附分子（CAM）、血管内皮生长因子（VEGF）信号通路和丝裂原活化蛋白激酶（MAPK）信号通路有关。这些结果为[鱼的种类未明确]适应缺氧应激的生理和生化机制提供了新的见解。此外，这些结果为未来研究缺氧耐受性的分子机制以及[鱼的种类未明确]和其他鱼类的健康养殖提供了框架。
