Zhu Fei, Lei Meixuan, Song Yufeng, Xu Dafeng, Du Shuran, Meng Qian, Jia Chaofeng, Yin Shaowu, Chen Shuyin, Zhao Cheng
Jiangsu Marine Fisheries Research Institute, Nantong, Jiangsu, China.
College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu, China.
Fish Physiol Biochem. 2025 Apr 7;51(2):79. doi: 10.1007/s10695-025-01492-0.
The silver pomfret (Pampus argenteus) is an economically valuable and highly sought-after table fish in China. In recent years, commercial farming of silver pomfret has been successfully developed in the country. However, silver pomfret is highly sensitive to environmental stress, and hypoxia-induced stress can lead to significant economic losses in aquaculture. This study investigated the transcriptomic response of silver pomfret gills under normal oxygen conditions (G0) and after hypoxic exposure for 6 h (G6) and 24 h (G24). Hypoxia exposure induced gill remodeling, characterized by increased gill lamellar height and a reduction in interlamellar cell mass (ILCM). Oxidative stress and antioxidant responses were significantly upregulated after 24 h of hypoxia exposure. Additionally, many downregulated genes were significantly enriched in pathways related to cardiac muscle contraction and calcium signaling, leading to impaired gill musculature contraction and reduced oxygen uptake under hypoxic conditions. Key signal transduction pathways, including HIF- 1, Apelin, and MAPK signaling, were identified as critical pathways in response to hypoxia. Furthermore, hypoxia tended to suppress the immune system and disrupted endoplasmic reticulum homeostasis and protein processing in the gills of silver pomfret. In summary, this study demonstrates that hypoxia disrupts gill function in silver pomfret and provides insights into hypoxia adaptation mechanisms in teleosts.
银鲳（Pampus argenteus）是中国一种具有经济价值且备受青睐的食用鱼。近年来，该国已成功发展了银鲳的商业养殖。然而，银鲳对环境压力高度敏感，缺氧诱导的应激会导致水产养殖遭受重大经济损失。本研究调查了正常氧气条件下（G0）以及缺氧暴露6小时（G6）和24小时（G24）后银鲳鳃的转录组反应。缺氧暴露诱导鳃重塑，其特征为鳃小片高度增加和片间细胞团（ILCM）减少。缺氧暴露24小时后，氧化应激和抗氧化反应显著上调。此外，许多下调基因在与心肌收缩和钙信号传导相关的通路中显著富集，导致缺氧条件下鳃肌肉组织收缩受损和氧气摄取减少。关键信号转导通路，包括HIF-1、Apelin和MAPK信号通路，被确定为对缺氧反应的关键通路。此外，缺氧倾向于抑制免疫系统，并破坏银鲳鳃中的内质网稳态和蛋白质加工。总之，本研究表明缺氧会破坏银鲳的鳃功能，并为硬骨鱼的缺氧适应机制提供了见解。
