Zu Xiao-Yan, Liu Wen-Bo, Xiong Guang-Quan, Liao Tao, Li Hai-Lan
Key Laboratory of Cold Chain Logistics Technology for Agro-Product (Ministry of Agriculture and Rural Affairs), Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
School of Chemical and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430205, China.
Foods. 2023 May 9;12(10):1934. doi: 10.3390/foods12101934.
Swim bladder polypeptides (SBPs) of were analyzed for their antioxidant activity and physicochemical properties. The results showed the optimal enzymatic conditions were alkaline protease with a solid-to-liquid ratio of 1:20, an incubation time of 4 h, a temperature of 55 °C, and an enzyme dosage of 5000 U/g. Three different molecular weight fractions (F1, F2, and F3) were obtained via ultrafiltration. F3 (912.44-2135.82 Da) showed 77.90%, 72.15%, and 66.25% removal of O•, DPPH•, and •OH, respectively, at 10 mg/mL, which was significantly higher than the F1 and F2 fractions ( < 0.05). F3 contained proline (6.17%), hydroxyproline (5.28%), and hydrophobic amino acids (51.39%). The UV spectrum of F3 showed maximum absorption at 224 nm. Peptide sequence analysis showed that F3 contained antioxidant peptides (MFGF, GPPGPRGPPGL, and GPGPSGERGPPGPM) and exhibited inhibitory activities on angiotensin-converting enzyme and dipeptidyl peptidase III/IV (FRF, FPFL and LPGLF). F3 was considered a good raw material for obtaining bioactive peptides.
对鱼鳔多肽（SBPs）的抗氧化活性和理化性质进行了分析。结果表明，最佳酶解条件为碱性蛋白酶，料液比1:20，孵育时间4 h，温度55℃，酶用量5000 U/g。通过超滤获得了三种不同分子量的组分（F1、F2和F3）。F3（912.44 - 2135.82 Da）在10 mg/mL时对超氧阴离子（O•）、二苯代苦味酰基自由基（DPPH•）和羟基自由基（•OH）的清除率分别为77.90%、72.15%和66.25%，显著高于F1和F2组分（P < 0.05）。F3含有脯氨酸（6.17%）、羟脯氨酸（5.28%）和疏水氨基酸（51.39%）。F3的紫外光谱在224 nm处有最大吸收。肽序列分析表明，F3含有抗氧化肽（MFGF、GPPGPRGPPGL和GPGPSGERGPPGPM），并对血管紧张素转换酶和二肽基肽酶III/IV具有抑制活性（FRF、FPFL和LPGLF）。F3被认为是获得生物活性肽的良好原料。
