Department of Food Science and Technology, College of Biomass and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Food Funct. 2022 Jun 6;13(11):6008-6021. doi: 10.1039/d2fo00729k.
Ocean life contains a wealth of bioactive peptides that could be utilized in nutraceuticals and pharmaceuticals. This study aimed to obtain neuroprotective antioxidant peptides in sea squirt () through protamex enzymolysis. Fraction F4 (ultrafiltration generated four fractions) had a lower molecular weight (<500 Dalton (Da)) with greater 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities (94.24 ± 2.50% and 91.80 ± 1.19%). After gel filtration, six peptides, including Phe-Gly-Phe (FGF), Leu-Gly-Phe (LGF), Leu-Phe-VAL (LFV), Val-Phe-Leu (VFL), Trp-Leu-Pro (WLP), and Ile-Ser-Trp (ISW), were identified and sequenced by liquid chromatography-mass spectrometry (LC-MS/MS). Peptides WLP and ISW showed higher oxygen radical absorbance capacity (ORAC) values (2.72 ± 0.47 and 1.93 ± 0.01 μmol L of Trolox equivalent (TE) per μmol L of peptide) than glutathione (GSH). Additionally, WLP effectively increased cell viability, dramatically attenuated 6-Hydroxydopamine (6-OHDA)-induced cell apoptosis and decreased reactive oxygen species (ROS) levels to nearly two-fold, and significantly boosted glutathione peroxidase (GSH-Px) activity in PC12 cells. Transcriptome sequencing revealed differential expression of genes associated with various oxidative stress pathways after WLP treatment, such as glutathione metabolism. These results suggest that the -derived tripeptide WLP could alleviate neurodegenerative diseases associated with oxidative stress.
海洋生物蕴含着丰富的具有生物活性的肽，可应用于营养保健品和药物领域。本研究旨在通过酶解法从海鞘中获得具有神经保护作用的抗氧化肽。F4 级分（超滤生成的四个级分之一）的分子量较低（<500 道尔顿（Da）），对 1,1-二苯基-2-苦基肼（DPPH）和 2,2-连氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基的清除能力更强（94.24±2.50%和 91.80±1.19%）。经过凝胶过滤后，通过液相色谱-质谱联用技术（LC-MS/MS）共鉴定并测序了 6 种肽，分别为苯丙氨酸-甘氨酸-苯丙氨酸（FGF）、亮氨酸-甘氨酸-苯丙氨酸（LGF）、亮氨酸-苯丙氨酸-缬氨酸（LFV）、缬氨酸-苯丙氨酸-亮氨酸（VFL）、色氨酸-亮氨酸-脯氨酸（WLP）和异亮氨酸-丝氨酸-色氨酸（ISW）。肽 WLP 和 ISW 的氧自由基吸收能力（ORAC）值（2.72±0.47 和 1.93±0.01 μmol L 三羟甲基氨基甲烷（Tris）等效物（TE）/μmol L 肽）高于谷胱甘肽（GSH）。此外，WLP 能有效提高 PC12 细胞活力，显著减轻 6-羟基多巴胺（6-OHDA）诱导的细胞凋亡，使活性氧（ROS）水平降低近两倍，并显著提高 PC12 细胞的谷胱甘肽过氧化物酶（GSH-Px）活性。转录组测序结果表明，WLP 处理后，与谷胱甘肽代谢等多种氧化应激途径相关的基因表达存在差异。这些结果表明，来源于海鞘的三肽 WLP 可缓解与氧化应激相关的神经退行性疾病。
