Zhao Linfen, Wang Yanhua, Wang Dongyu, He Zejuan, Gong Jiashun, Tan Chao
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Agro-Products Processing Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650201, China.
Foods. 2023 May 16;12(10):2015. doi: 10.3390/foods12102015.
Headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) was used to study the effects of four kinds of probiotics on the volatile components of fermented coffee. The fingerprints showed that 51 compounds were confirmed and quantified, including 13 esters, 11 aldehydes, 9 alcohols, 6 ketones, 3 furans, 5 terpenes (hydrocarbons), 2 organic acids, 1 pyrazine, and 1 sulfur-containing compound. After fermenting, the aroma of the green beans increases while that of the roasted beans decreases. After roasting, the total amount of aroma components in coffee beans increased by 4.48-5.49 times. The aroma differences between fermented and untreated roasted beans were more significant than those between fermented and untreated green beans. HS-GC-IMS can distinguish the difference in coffee aroma, and each probiotic has a unique influence on the coffee aroma. Using probiotics to ferment coffee can significantly improve the aroma of coffee and provide certain application prospects for improving the quality of commercial coffee beans.
顶空-气相色谱-离子迁移谱(HS-GC-IMS)被用于研究四种益生菌对发酵咖啡挥发性成分的影响。指纹图谱显示,共确认并定量了51种化合物,包括13种酯类、11种醛类、9种醇类、6种酮类、3种呋喃类、5种萜类(烃类)、2种有机酸、1种吡嗪类和1种含硫化合物。发酵后,生豆的香气增加,而烘焙豆的香气减少。烘焙后,咖啡豆中香气成分的总量增加了4.48-5.49倍。发酵烘焙豆与未处理烘焙豆之间的香气差异比发酵生豆与未处理生豆之间的差异更显著。HS-GC-IMS能够区分咖啡香气的差异,每种益生菌对咖啡香气都有独特的影响。使用益生菌发酵咖啡可以显著改善咖啡的香气,并为提高商业咖啡豆的品质提供一定的应用前景。