Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.
Genes (Basel). 2023 Apr 30;14(5):1030. doi: 10.3390/genes14051030.
Faba bean is an important legume crop consumed as a vegetable or snack food, and its green cotyledons could present an attractive color for consumers. A mutation in causes stay-green in plants. In this study, was identified from a green-cotyledon-mutant faba bean, SNB7, by homologous blast between the SGR of pea and the transcriptome of faba bean. Sequence analysis revealed that a SNP at position 513 of the CDS of caused a pre-stop codon, resulting in a shorter protein in the green-cotyledon faba bean SNB7. A dCaps marker was developed according to the SNP that caused the pre-stop, and this marker was completely associated with the color of the cotyledon of faba bean. SNB7 stayed green during dark treatment, while the expression level of increased during dark-induced senescence in the yellow-cotyledon faba bean HST. Transient expression of in leaves resulted in chlorophyll degradation. These results indicate that is the gene responsible for the stay-green of faba bean, and the dCaps marker developed in this study provides a molecular tool for the breeding of green-cotyledon faba beans.
蚕豆是一种重要的豆类作物，可作为蔬菜或零食食用，其绿色子叶对消费者具有吸引力。突变导致植物保持绿色。本研究通过豌豆 SGR 和蚕豆转录组之间的同源性比对，从绿色子叶突变体蚕豆 SNB7 中鉴定出。序列分析表明，CDS 第 513 位的 SNP 导致提前终止密码子，导致绿色子叶蚕豆 SNB7 中的蛋白质变短。根据导致提前终止的 SNP 开发了 dCaps 标记，该标记与蚕豆子叶的颜色完全相关。蚕豆 SNB7 在黑暗处理期间保持绿色，而黄色子叶蚕豆 HST 在黑暗诱导衰老期间 表达水平增加。在 叶片中转录瞬时表达导致叶绿素降解。这些结果表明 是导致蚕豆保持绿色的基因，本研究中开发的 dCaps 标记为培育绿色子叶蚕豆提供了分子工具。
