解析高风险 ST147 克隆的进化动态：比较泛基因组分析的见解。

Unravelling the Evolutionary Dynamics of High-Risk ST147 Clones: Insights from Comparative Pangenome Analysis.

机构信息

Centre for Biotechnology, School of Pharmaceutical Sciences, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar 751003, India.

Department of Microbiology, University of Manitoba, Winnipeg, MB R3T2N2, Canada.

出版信息

Genes (Basel). 2023 May 2;14(5):1037. doi: 10.3390/genes14051037.

BACKGROUND

The high prevalence and rapid emergence of antibiotic resistance in high-risk ST147 clones is a global health concern and warrants molecular surveillance.

METHODS

A pangenome analysis was performed using publicly available ST147 complete genomes. The characteristics and evolutionary relationships among ST147 members were investigated through a Bayesian phylogenetic analysis.

RESULTS

The large number of accessory genes in the pangenome indicates genome plasticity and openness. Seventy-two antibiotic resistance genes were found to be linked with antibiotic inactivation, efflux, and target alteration. The exclusive detection of the gene within the ColKp3 plasmid of KP_SDL79 suggests its acquisition through horizontal gene transfer. The association of seventy-six virulence genes with the efflux pump, T6SS system and type I secretion system describes its pathogenicity. The presence of Tn, a putative Tn7-like transposon in KP_SDL79 with an insertion at the flanking region of the gene, establishes its transmission ability. The Bayesian phylogenetic analysis estimates ST147's initial divergence in 1951 and the most recent common ancestor for the entire population in 1621.

CONCLUSIONS

Present study highlights the genetic diversity and evolutionary dynamics of high-risk clones of . Further inter-clonal diversity studies will help us understand its outbreak more precisely and pave the way for therapeutic interventions.

背景

高危 ST147 克隆中抗生素耐药性的高流行率和快速出现是一个全球健康关注问题，需要进行分子监测。

方法

使用公开的 ST147 全基因组进行泛基因组分析。通过贝叶斯系统发育分析研究 ST147 成员之间的特征和进化关系。

结果

泛基因组中的大量辅助基因表明了基因组的可塑性和开放性。发现 72 个抗生素耐药基因与抗生素失活、外排和靶标改变有关。在 KP_SDL79 的 ColKp3 质粒中仅检测到基因，表明其通过水平基因转移获得。76 个毒力基因与外排泵、T6SS 系统和 I 型分泌系统相关，描述了其致病性。在 KP_SDL79 中存在 Tn，一种假定的 Tn7 样转座子，在基因侧翼区域有一个插入，建立了其传播能力。贝叶斯系统发育分析估计 ST147 的初始分歧发生在 1951 年，整个种群的最近共同祖先发生在 1621 年。