Analysis of Promoter Activity and Screening of Related Transcription Factors in Dolang Sheep.

The gene is involved in the initiation of puberty, but its regulatory mechanisms remain unclear. Therefore, in this study, we aimed to study the regulatory mechanism of the promoter by cloning the proximal promoter for bioinformatic analysis. Next, a series of deletion vectors were constructed based on the bioinformatic analysis results for dual-fluorescein activity detection. The transcriptional regulation mechanism of the promoter region was analyzed by detecting mutations in transcription factor-binding sites and overexpression of transcription factors. The dual-luciferase assay showed that the promoter region -837 to -338 bp had the highest transcriptional activity, and the transcriptional activity of the transcriptional regulatory region decreased significantly after and mutations. Overexpression of the Egr1 transcription factor significantly enhanced the transcription of , and the results indicated that and play important roles in regulating . These results provide a theoretical basis for further research on the transcriptional regulation of sheep during puberty initiation.