Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Int J Mol Sci. 2023 May 9;24(10):8501. doi: 10.3390/ijms24108501.
This study was conducted to evaluate the effects of a low-protein (LP) diet supplemented with sodium butyrate (SB), medium-chain fatty acids (MCFAs) and n-3 polyunsaturated fatty acids (PUFAs) on nutrient utilization and lipid and amino acid metabolism in weaned pigs. A total of 120 Duroc × Landrace × Yorkshire pigs (initial body weight: 7.93 ± 0.65 kg) were randomly assigned to five dietary treatments, including the control diet (CON), LP diet, LP + 0.2% SB diet (LP + SB), LP + 0.2% MCFA diet (LP + MCFA) and LP + 0.2% n-3 PUFA diet (LP + PUFA). The results show that the LP + MCFA diet increased ( < 0.05) the digestibility of dry matter and total P in pigs compared with the CON and LP diets. In the liver of the pigs, the metabolites involved in sugar metabolism and oxidative phosphorylation significantly changed with the LP diet compared with the CON diet. Compared with the LP diet, the altered metabolites in the liver of the pigs fed with the LP + SB diet were mainly associated with sugar metabolism and pyrimidine metabolism; the altered metabolites in the liver of pigs fed with the LP + MCFA and LP + PUFA diets were mainly associated with lipid metabolism and amino acid metabolism. In addition, the LP + PUFA diet increased ( < 0.05) the concentration of glutamate dehydrogenase in the liver of pigs compared with the LP diet. Furthermore, the LP + MCFA and LP + PUFA diets increased ( < 0.05) the mRNA abundance of sterol regulatory element-binding protein 1 and acetyl-CoA carboxylase in the liver compared with the CON diet. The LP + PUFA diet increased ( < 0.05) mRNA abundances of fatty acid synthase in the liver compared with the CON and LP diets. Collectively, the LP diet supplemented with MCFAs improved nutrient digestibility, and the LP diet supplemented with MCFAs and n-3 PUFAs promoted lipid and amino acid metabolisms.
本研究旨在评估低蛋白（LP）饲料中添加丁酸钠（SB）、中链脂肪酸（MCFA）和 n-3 多不饱和脂肪酸（PUFA）对断奶仔猪养分利用及脂类和氨基酸代谢的影响。选用 120 头杜×长×大三元杂交仔猪（初始体重：7.93±0.65kg），随机分为 5 个处理组，分别为对照组（CON）、LP 组、LP+0.2%SB 组（LP+SB）、LP+0.2%MCFA 组（LP+MCFA）和 LP+0.2%n-3PUFA 组（LP+PUFA）。结果表明，与 CON 和 LP 组相比，LP+MCFA 组提高了仔猪对干物质和总磷的消化率（<0.05）。与 CON 组相比，LP 组仔猪肝脏中与糖代谢和氧化磷酸化相关的代谢物发生了显著变化。与 LP 组相比，LP+SB 组仔猪肝脏中发生变化的代谢物主要与糖代谢和嘧啶代谢有关；LP+MCFA 和 LP+PUFA 组仔猪肝脏中发生变化的代谢物主要与脂类代谢和氨基酸代谢有关。此外，与 LP 组相比，LP+PUFA 组提高了仔猪肝脏中谷氨酸脱氢酶的浓度（<0.05）。此外，与 CON 组相比，LP+MCFA 和 LP+PUFA 组增加了肝脏中固醇调节元件结合蛋白 1 和乙酰辅酶 A 羧化酶的 mRNA 丰度（<0.05）。与 CON 和 LP 组相比，LP+PUFA 组增加了肝脏中脂肪酸合酶的 mRNA 丰度（<0.05）。总之，LP 饲料中添加 MCFA 可提高养分消化率，LP 饲料中添加 MCFA 和 n-3PUFA 可促进脂类和氨基酸代谢。
