Albaqami Fahad F, Althurwi Hassan N, Alharthy Khalid M, Hamad Abubaker M, Awartani Fatin A
Postgraduate Doctorate Program, Periodontics and Community Dentistry Department, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia.
Pharmacology and Toxicology Department, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.
Pharmaceuticals (Basel). 2023 May 22;16(5):774. doi: 10.3390/ph16050774.
Bone graft techniques are used to compensate for bone loss in areas with deficient regeneration. However, matrix metalloproteases (MMPs) can limit bone formation by degrading extracellular matrices, which are required for bone regrowth. Noteworthily, rutin is a natural flavonoid compound that inhibits the genetic expression of various MMPs. Therefore, rutin may serve as an inexpensive and stable alternative to the growth factors used to accelerate dental bone graft healing. This study aimed to evaluate the potential of mixing rutin gel with allograft bone to accelerate the healing of bone defects in an in vivo rabbit model. Bone defects were surgically induced in New Zealand rabbits ( = 3 per group) and subsequently treated with bone grafts along with rutin or control gel. Overall, treatment with rutin significantly prevented the expression of several MMPs and increased type III collagen in the gingiva around the surgical site. Additionally, rutin-treated animals showed enhanced bone formation with higher bone marrow content in the jawbone defect area compared with the control group. Taken together, these findings demonstrate that rutin gel, when added to bone grafts, quickly enhances bone formation and may serve as a suitable alternative to expensive growth factors for the same purpose.
骨移植技术用于补偿再生不足区域的骨丢失。然而，基质金属蛋白酶（MMPs）可通过降解骨再生所需的细胞外基质来限制骨形成。值得注意的是，芦丁是一种天然黄酮类化合物，可抑制多种MMPs的基因表达。因此，芦丁可作为用于加速牙骨移植愈合的生长因子的一种廉价且稳定的替代品。本研究旨在评估将芦丁凝胶与同种异体骨混合在体内兔模型中加速骨缺损愈合的潜力。在新西兰兔（每组 = 3只）中手术诱导骨缺损，随后用骨移植材料联合芦丁或对照凝胶进行治疗。总体而言，芦丁治疗显著抑制了几种MMPs的表达，并增加了手术部位周围牙龈中III型胶原蛋白的含量。此外，与对照组相比，接受芦丁治疗的动物在颌骨缺损区域显示出增强的骨形成，骨髓含量更高。综上所述，这些发现表明，当添加到骨移植材料中时，芦丁凝胶可快速促进骨形成，并可作为用于相同目的的昂贵生长因子的合适替代品。
