Younis Tahira, Jabeen Faiza, Hussain Ali, Rasool Bilal, Raza Ishaq Ali, Nawaz Atif, El-Nashar Heba A S, El-Shazly Mohamed
Department of Biochemistry and Biotechnology, The Women University Multan, 60000, Multan, Pakistan.
Department of Biochemistry, Quaid-i-Azam University, 45320, Islamabad, Pakistan.
Chem Biodivers. 2023 Mar;20(3):e202200755. doi: 10.1002/cbdv.202200755. Epub 2023 Feb 24.
Fraxinus xanthoxyloides is a perennial shrub belonging to family Oleaceae, traditionally used for malaria, jaundice, pneumonia, inflammation, and rheumatism. Our study is aimed to assess the total phenolics (TPC), flavonoids (TFC), terpenoids contents (TTC) and antioxidant profiling of F. xanthoxyloides methanol bark extract (FXBM) and its fractions, hexane, chloroform, ethyl acetate and aqueous, along with high-performance liquid chromatography with diode-array detection (HPLC-DAD). Further, the antioxidant and pulmonary protective potential was explored against carbon tetrachloride (CCl )-induced CCl4-induced pulmonary tissue damage in rats. The highest TPC, TFC and TTC were found in FXBM (133.29±4.19 mg/g), ethyl acetate fraction (279.55±10.35 mg/g), and chloroform fraction (0.79±0.06 mg/g), respectively. The most potent antioxidant capacity was depicted by FXBM (29.21±2.40 μg/mg) and ethyl acetate fraction (91.16±5.51 μg/mg). The HPLC-DAD analysis revealed the predominance of gallic, chlorogenic, vanillic and ferulic acid in FXBM. The administration of CCl induced oxidative stress, suppressed antioxidant enzymes' activities including catalase, peroxidase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, and glutathione reductase. Further, it increased thiobarbituric acid reactive substances (TBARS) and H O levels, induced DNA injuries and reduced the total protein and glutathione content in lung tissues. The treatment of rats with FXBM restored these biochemical parameters to the normal level. Moreover, the histopathological studies of lung tissues demonstrated that FXBM protected rats' lung tissues from oxidative damage restoring normal lung functions. Thus, F. xanthoxyloides bark extract is recommended as adjuvant therapy as protective agent for patients with lung disorders.
清香木犀是一种属于木犀科的多年生灌木，传统上用于治疗疟疾、黄疸、肺炎、炎症和风湿病。我们的研究旨在评估清香木犀甲醇树皮提取物（FXBM）及其己烷、氯仿、乙酸乙酯和水相馏分中的总酚（TPC）、黄酮类化合物（TFC）、萜类化合物含量（TTC）和抗氧化特性，同时采用二极管阵列检测高效液相色谱法（HPLC-DAD）进行分析。此外，还研究了其对四氯化碳（CCl₄）诱导的大鼠肺组织损伤的抗氧化和肺保护潜力。结果发现，FXBM中TPC最高（133.29±4.19 mg/g），乙酸乙酯馏分中TFC最高（279.55±10.35 mg/g），氯仿馏分中TTC最高（0.79±0.06 mg/g）。FXBM（29.21±2.40 μg/mg）和乙酸乙酯馏分（91.16±5.51 μg/mg）表现出最强的抗氧化能力。HPLC-DAD分析显示，FXBM中没食子酸、绿原酸、香草酸和阿魏酸占主导地位。给予CCl₄会诱导氧化应激，抑制过氧化氢酶、过氧化物酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶和谷胱甘肽还原酶等抗氧化酶的活性。此外，它还会增加硫代巴比妥酸反应性物质（TBARS）和H₂O₂水平，诱导DNA损伤，并降低肺组织中的总蛋白和谷胱甘肽含量。用FXBM治疗大鼠可使这些生化参数恢复到正常水平。此外，肺组织的组织病理学研究表明，FXBM可保护大鼠肺组织免受氧化损伤，恢复正常肺功能。因此，建议将清香木犀树皮提取物作为辅助治疗药物，作为肺部疾病患者的保护剂。
