de Herder W W, Hazenberg M P, Pennock-Schröder A M, Hennemann G, Visser T J
Med Biol. 1986;64(1):31-5.
Faecal suspensions from healthy humans, conventional (CV), germ-free (GF) and intestine-decontaminated (ID) rats were tested for the in vitro hydrolysis of 125I-labelled iodothyronine sulphates and 3,3',5-triiodothyronine glucuronide (T3G). Whereas 20-fold diluted human and CV rat faecal suspensions hydrolyzed up to 90% of the sulphates, no hydrolysis was observed in 5 times diluted faecal suspensions of GF and ID rats. These results add further weight to the assumption that intestinal iodothyronine sulphatase activity is of bacterial origin. Twenty times diluted human and CV rat faecal suspensions hydrolyzed approximately 80% of the T3G. In the 5 times diluted faecal suspensions of GF and ID rats up to 15% hydrolysis of T3G was still observed. It was concluded that the major part of the gastrointestinal iodothyronine glucuronidase activity is produced by bacteria. The remaining activity presumably originates from gastrointestinal mucosal cells.
对来自健康人类、常规饲养（CV）、无菌（GF）和肠道去污处理（ID）大鼠的粪便悬液进行了体外实验，以检测其对125I标记的碘甲状腺原氨酸硫酸盐和3,3',5-三碘甲状腺原氨酸葡萄糖醛酸苷（T3G）的水解能力。20倍稀释的人类和CV大鼠粪便悬液可水解高达90%的硫酸盐，而5倍稀释的GF和ID大鼠粪便悬液中未观察到水解现象。这些结果进一步支持了肠道碘甲状腺原氨酸硫酸酯酶活性起源于细菌的假设。20倍稀释的人类和CV大鼠粪便悬液可水解约80%的T3G。在5倍稀释的GF和ID大鼠粪便悬液中，仍观察到高达15%的T3G水解。得出的结论是，胃肠道碘甲状腺原氨酸葡萄糖醛酸苷酶活性的主要部分由细菌产生。其余活性可能起源于胃肠道黏膜细胞。
