Greer E R, Caldwell J D, Johnson M F, Prange A J, Pedersen C A
Life Sci. 1986 Jun 23;38(25):2311-8. doi: 10.1016/0024-3205(86)90638-7.
Oxytocin (OT) and arginine-8-vasopressin (AVP) were measured by radioimmunoassay in micropunched hypothalamic neurosecretory nuclei of estrous cycling female Sprague-Dawley rats. In the paraventricular nucleus (PVN): the concentration (pg/microgram protein) of OT was significantly higher in rats in diestrus than during proestrus, estrus, or metestrus, while the concentration during metestrus was significantly greater than in proestrus and estrus; the concentration of AVP was significantly lower in animals in estrus than during the other three stages; because the paraventricular OT levels dropped before proestrus, the AVP/OT ratio was significantly greater in animals in proestrus than in diestrus, metestrus, and estrus. In the supraoptic nucleus (SON) a similar trend was noted: the concentration of OT was highest during diestrus, and AVP was lowest during estrus, though neither was significantly different from other stages. Because the OT and AVP cycles in the SON were asynchronous, the ratio of AVP to OT was significantly higher in proestrus than in metestrus or diestrus and significantly greater in estrus than during diestrus. In contrast to these two areas, peptide concentrations did not vary significantly across the estrous cycle in other sites of nonapeptide synthesis, i.e. the anterior commissural nucleus (ACN) and the suprachiasmatic nuclei (SCN).
采用放射免疫分析法，对处于发情周期的雌性斯普拉格-道利大鼠经微量打孔获取的下丘脑神经分泌核团中的催产素（OT）和精氨酸加压素（AVP）进行了测定。在室旁核（PVN）中：处于间情期的大鼠OT浓度（pg/微克蛋白）显著高于发情前期、发情期或发情后期，而发情后期的浓度显著高于发情前期和发情期；发情期动物的AVP浓度显著低于其他三个阶段；由于发情前期室旁核OT水平下降，发情前期动物的AVP/OT比值显著高于间情期、发情后期和发情期。在视上核（SON）中也观察到类似趋势：间情期OT浓度最高，发情期AVP浓度最低，不过与其他阶段相比均无显著差异。由于视上核中OT和AVP的周期不同步，发情前期AVP与OT的比值显著高于发情后期或间情期，发情期显著高于间情期。与这两个区域不同，在其他九肽合成部位，即前连合核（ACN）和视交叉上核（SCN），肽浓度在发情周期中无显著变化。
