Ho M L, Lee J N
Department of Physiology, Kaohsiung Medical College, Taiwan, ROC.
Acta Endocrinol (Copenh). 1992 Jun;126(6):530-4. doi: 10.1530/acta.0.1260530.
Ovarian extracts of Long-Evans rats separated using high performance liquid chromatography (HPLC) were measured by radioimmunoassays (RIAs) for the presence of oxytocin and arginine vasopressin (AVP). The results showed that the ovary contains both, and that they are indistinguishable from the respective standard synthetic peptides. During the estrous cycle, the ovarian content of oxytocin was 10-fold higher (p less than 0.01) in estrus than in the other phases, while AVP was 16- and 25-fold higher (p less than 0.01) in metestrus than in the other phases. In contrast, the plasma levels of oxytocin showed no significant difference among the various phases of the estrous cycle. However, the plasma level of AVP level was significantly higher (p less than 0.01) in diestrus than in other phases. The present study thus strongly supports the hypothesis that both oxytocin and AVP can be produced by the ovary itself in the rat. The possible roles of oxytocin and AVP in the reproductive cycle are discussed.
采用高效液相色谱法（HPLC）分离长 Evans 大鼠的卵巢提取物，并用放射免疫分析法（RIA）检测其中催产素和精氨酸加压素（AVP）的存在情况。结果显示，卵巢中同时含有这两种物质，且它们与各自的标准合成肽无法区分。在发情周期中，发情期卵巢中催产素的含量比其他阶段高 10 倍（p < 0.01），而在动情后期 AVP 的含量比其他阶段分别高 16 倍和 25 倍（p < 0.01）。相比之下，发情周期各阶段血浆中催产素水平无显著差异。然而，动情间期血浆中 AVP 水平显著高于其他阶段（p < 0.01）。因此，本研究有力地支持了大鼠卵巢自身能够产生催产素和 AVP 的假说。文中还讨论了催产素和 AVP 在生殖周期中的可能作用。
