CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, 130000, China.
Antiviral Res. 2023 Jul;215:105644. doi: 10.1016/j.antiviral.2023.105644. Epub 2023 May 25.
African swine fever (ASF) is a highly contagious and deadly disease that affects domestic and wild pigs. No commercial vaccine or antiviral is currently available against ASF. The control of ASF primarily relies on implementing effective biosecurity measures during the breeding process. Here, we evaluated the preventive and therapeutic potential of the interferon (IFN) cocktail (a mixture of recombinant porcine IFN α and γ) on ASF. The IFN cocktail treatment delayed the onset of ASF symptoms and ASF virus (ASFV) replication for approximately one week. However, IFN cocktail treatment could not prevent the death of the pigs. Further analysis showed that IFN cocktail treatment increased the expression of multiple IFN-stimulated genes (ISGs) in porcine peripheral blood mononuclear cells in vivo and in vitro. Additionally, IFN cocktail modulated the expression of pro- and anti-inflammatory cytokines and reduced tissue injury in the ASFV-infected pigs. Collectively, the results suggest that the IFN cocktail restricts the progression of acute ASF by inducing high levels of ISGs, contributing to the pre-establishment of antiviral status, and modulating the balance of pro- and anti-inflammatory mediators to lessen cytokine storm-mediated tissue damage.
非洲猪瘟（ASF）是一种高度传染性和致命性疾病，会影响家猪和野猪。目前尚无针对 ASF 的商业疫苗或抗病毒药物。ASF 的控制主要依赖于在养殖过程中实施有效的生物安全措施。在这里，我们评估了干扰素（IFN）鸡尾酒（重组猪 IFNα和γ的混合物）对 ASF 的预防和治疗潜力。IFN 鸡尾酒治疗可将 ASF 症状和 ASF 病毒（ASFV）复制的发作时间延迟约一周。然而，IFN 鸡尾酒治疗并不能阻止猪的死亡。进一步的分析表明，IFN 鸡尾酒治疗可在体内和体外增加猪外周血单核细胞中多种干扰素刺激基因（ISGs）的表达。此外，IFN 鸡尾酒可调节感染 ASFV 的猪中的促炎和抗炎细胞因子的表达，并减轻组织损伤。总之，结果表明 IFN 鸡尾酒通过诱导高水平的 ISGs 来限制急性 ASF 的进展，有助于预先建立抗病毒状态，并调节促炎和抗炎介质的平衡，以减轻细胞因子风暴介导的组织损伤。
