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猪干扰素混合物可降低死亡率，并减轻非洲猪瘟病毒感染仔猪所造成的组织损伤。

Swine IFN cocktail can reduce mortality and lessen the tissue injury caused by African swine-fever-virus-infected piglets.

作者信息

Jiang Yitong, Jiang Fei, Zhai Wenzhu, Huang Ying, Pang Zhongbao, Tao Chunhao, Wang Zhen, He Yuheng, Chu Yuanyuan, Zhu Hongfei, Wu Jiajun, Jia Hong

机构信息

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

China Animal Disease Control Center, Beijing, China.

出版信息

Front Cell Infect Microbiol. 2024 Dec 3;14:1388035. doi: 10.3389/fcimb.2024.1388035. eCollection 2024.

DOI:10.3389/fcimb.2024.1388035
PMID:39691698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11649406/
Abstract

African swine fever (ASF), a highly virulent viral infection, poses a significant threat to the global pig industry. Currently, there are no commercially available vaccines against ASF. While the crucial role of interferon (IFN) in combating viral infections is well-established, its impact on the clinical signs and mortality rates of ASF remains unclear. In this study, swine IFN-α2, IFN-γ, and IFN-λ3 were fused with the Fc segment of immunoglobulin G (IgG) and expressed in mammalian cells (293T), and the antiviral efficacy were detected by VSV-3D4/2 and VSV-PK15 systems. Then, the interferon stimulating genes (ISGs) induced by IFNs-hFc in 3D4/2 cells were determined by qRT-PCR. Also, the preventive potential of the interferon (IFN) cocktail (a mixture of IFNα2-hFc, IFNγ-hFc, and IFNλ3-hFc) were evaluated by 25-day-old piglets. The results showed that the specific activities of IFNα2-hFc, IFNγ-hFc, and IFNλ3-hFc were 2.46 × 10 IU/mL, 4.54 × 10 IU/mL and 7.54 × 10 IU/mL, respectively. The IFN-hFc significantly induced the expression of various IFN-stimulated genes (ISGs) in 3D4/2 cells after IFNs-Fc treatment, including , , , , , , , , and . Furthermore, the IFN cocktail treatment reduced the viral load, delayed death, and reduced tissue injury in the piglets infected with ASF virus (ASFV). in conclusion, these results suggest that the IFNs-hFc showed high anti-viral activity, and the IFN cocktail may be potential for the prevention and treatment of ASF.

摘要

非洲猪瘟（ASF）是一种高致病性病毒感染，对全球养猪业构成重大威胁。目前，尚无针对ASF的商业化可用疫苗。虽然干扰素（IFN）在对抗病毒感染中的关键作用已得到充分证实，但其对ASF临床症状和死亡率的影响仍不清楚。在本研究中，将猪IFN-α2、IFN-γ和IFN-λ3与免疫球蛋白G（IgG）的Fc片段融合，并在哺乳动物细胞（293T）中表达，通过VSV-3D4/2和VSV-PK15系统检测抗病毒效果。然后，通过qRT-PCR确定IFNs-hFc在3D4/2细胞中诱导的干扰素刺激基因（ISGs）。此外，还对25日龄仔猪评估了干扰素（IFN）混合物（IFNα2-hFc、IFNγ-hFc和IFNλ3-hFc的混合物）的预防潜力。结果表明，IFNα2-hFc、IFNγ-hFc和IFNλ3-hFc的比活性分别为2.46×10 IU/mL、4.54×10 IU/mL和7.54×10 IU/mL。IFN-hFc在IFNs-Fc处理后显著诱导了3D4/2细胞中各种干扰素刺激基因（ISGs）的表达，包括 、 、 、 、 、 、 、 和 。此外，IFN混合物处理降低了感染非洲猪瘟病毒（ASFV）仔猪的病毒载量、延迟了死亡并减轻了组织损伤。总之，这些结果表明IFNs-hFc具有高抗病毒活性，且IFN混合物可能对ASF的预防和治疗具有潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/523689f341f6/fcimb-14-1388035-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/9d5fe9aaa667/fcimb-14-1388035-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/91afce275251/fcimb-14-1388035-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/fc075b52a915/fcimb-14-1388035-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/379eb486b69e/fcimb-14-1388035-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/709ac49eee64/fcimb-14-1388035-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/523689f341f6/fcimb-14-1388035-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/9d5fe9aaa667/fcimb-14-1388035-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/571f882fed32/fcimb-14-1388035-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/91afce275251/fcimb-14-1388035-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/fc075b52a915/fcimb-14-1388035-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/379eb486b69e/fcimb-14-1388035-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/709ac49eee64/fcimb-14-1388035-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e302/11649406/523689f341f6/fcimb-14-1388035-g007.jpg

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