辣根过氧化物酶抗体形成细胞及体液抗体的检测
Detection of antibody-forming cells and humoral antibody to horseradish peroxidase.
作者信息
Burke M, Harris D, Stollar B D, Borel H, Borel Y
出版信息
J Immunol Methods. 1979;25(4):365-74. doi: 10.1016/0022-1759(79)90028-0.
PMID:372446
Abstract
Two new simple methods for detecting antibody-forming cells by hemolytic plaque assay and hemagglutinating antibody to horseradish peroxidase have been developed in mice. Both techniques utilize as target, sheep erythrocytes coupled directly with horseradish peroxidase. These assays are sensitive, antigen-specific and are useful to quantitate both direct and indirect antibody-forming cells and humoral antibodies.
摘要
已在小鼠中开发出两种通过溶血空斑试验和抗辣根过氧化物酶血凝抗体检测抗体形成细胞的新的简单方法。这两种技术都将直接与辣根过氧化物酶偶联的绵羊红细胞用作靶标。这些试验灵敏、抗原特异性强，可用于定量直接和间接抗体形成细胞以及体液抗体。
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