用溶血空斑试验检测癌胚抗原抗体
Detection of antibody to carcinoembryonic antigen by the hemolytic plaque assay.
作者信息
Stinson R S, Lockhart C G, Margraf H W, Philpott G W
出版信息
Int Arch Allergy Appl Immunol. 1982;68(2):134-7. doi: 10.1159/000233082.
PMID:7042587
Abstract
A modification of the standard hemolytic plaque-forming cell assay is described. The conjugation of a soluble antigen, carcinoembryonic antigen, to the membrane of the sheep red blood cell was accomplished and used as the indicator system to detect antibody-forming cells. The specificity of the assay was determined by cross-reacting studies and competitive inhibition by antigen blocking.
摘要
本文描述了一种标准溶血空斑形成细胞试验的改良方法。将可溶性抗原癌胚抗原与绵羊红细胞膜进行偶联，并将其用作检测抗体形成细胞的指示系统。通过交叉反应研究和抗原阻断竞争抑制来确定该试验的特异性。
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