Hoogenraad N J, Wraight C J
Methods Enzymol. 1986;121:375-81. doi: 10.1016/0076-6879(86)21036-8.
Preinjection of mice 10 days before intraperitoneal injections of hybridomas gives optimum development of ascites tumors with respect to the rate at which tumors form, percentage of mice developing tumors, volume of ascites fluid produced, and concentration of monoclonal antibody in ascites fluid. The dose of pristane injected has little or no effect on ascites formation down to 0.1 ml of pristane.
在腹腔注射杂交瘤前10天对小鼠进行预注射，就肿瘤形成速率、发生肿瘤的小鼠百分比、产生的腹水体积以及腹水中单克隆抗体浓度而言，可使腹水肿瘤得到最佳发育。注射的 pristane 剂量低至0.1 ml 时，对腹水形成几乎没有影响。
