Hoogenraad N, Helman T, Hoogenraad J
J Immunol Methods. 1983 Jul 29;61(3):317-20. doi: 10.1016/0022-1759(83)90225-9.
Experiments were performed to determine whether for mice pre-injected with pristane (2,6,10,14-tetramethylpentadecane) there is an optimum interval before injection of hybridomas in order to maximize ascites fluid formation and yield of monoclonal antibodies. With injections of 0.5 ml of pristane followed by injections of 5 x 10(5) hybridoma cells making monoclonal antibodies against mammalian carbamyl phosphate synthetase, a pre-injection time of 10 days was optimum with respect to (a) the time taken for the ascites tumours to appear; (b) the percentage of mice developing ascites tumours (all mice developed tumours over a span of 2 days); and (c) the concentration of monoclonal antibodies in ascites fluid: 11 mg/ml compared with 7 mg/ml for the next highest group.
进行了实验，以确定对于预先注射了 pristane（2,6,10,14 - 四甲基十五烷）的小鼠，在注射杂交瘤之前是否存在一个最佳间隔时间，以便使腹水形成和单克隆抗体产量最大化。在注射 0.5 ml 的 pristane 后，接着注射 5×10⁵ 个针对哺乳动物氨甲酰磷酸合成酶产生单克隆抗体的杂交瘤细胞，就（a）腹水肿瘤出现所需的时间；（b）出现腹水肿瘤的小鼠百分比而言（所有小鼠在 2 天内都出现了肿瘤）；以及（c）腹水中单克隆抗体的浓度：为 11 mg/ml，而下一个最高组为 7 mg/ml，10 天的注射前时间是最佳的。
