Eager K B, Kennett R H
Methods Enzymol. 1986;121:59-69. doi: 10.1016/0076-6879(86)21009-5.
We have shown here that conventionally produced antisera aids in the production of specific monoclonal antibodies. The complex of immunoglobulin plus antigen acted as an effective immunogen in both rats and mice. Stable cell lines producing anit-human alpha 2M monoclonal antibodies to numerous antigenic sites on the alpha 2M molecule have been generated by this method. This method has also been used to produce monoclonal antibodies to human complement components. This procedure of immunizing mice with an immunoprecipitated product derived from conventionally produced antisera is a unique approach in that the antigen is conveniently enriched without tedious and time-consuming biochemical purification. In addition, the use of the immunoprecipitated product in conjunction with the immunoprecipitating antisera allows for rapid screening of the hybridomas in the initial stages when cell growth and maintenance is critical. This method thus further simplifies the task of obtaining a specific monoclonal antibody from a complex mixture. A final consideration is the wide availability of conventionally produced antisera to numerous proteins and other biological substances that could be used in the production of monoclonal antibodies, and consequently these antibodies could be used in more detailed studies of these same molecules.
我们在此表明，传统制备的抗血清有助于特定单克隆抗体的产生。免疫球蛋白与抗原的复合物在大鼠和小鼠中均作为有效的免疫原。通过这种方法已产生了针对α2M分子上众多抗原位点的抗人α2M单克隆抗体的稳定细胞系。该方法也已用于生产针对人补体成分的单克隆抗体。用源自传统制备抗血清的免疫沉淀产物免疫小鼠的这一程序是一种独特的方法，因为抗原无需繁琐且耗时的生化纯化即可方便地富集。此外，在细胞生长和维持至关重要的初始阶段，将免疫沉淀产物与免疫沉淀抗血清结合使用可快速筛选杂交瘤。因此，该方法进一步简化了从复杂混合物中获得特定单克隆抗体的任务。最后要考虑的是，传统制备的抗血清广泛可得，可用于众多蛋白质和其他生物物质的单克隆抗体制备，因此这些抗体可用于对这些相同分子的更详细研究。
