抗3,3',5-三碘-L-甲状腺原氨酸单克隆抗体的制备及部分特性鉴定

Production and partial characterization of monoclonal antibodies against 3,3',5-triiodo-L-thyronine.

作者信息

Marchand J, Gorce A, Badouaille G, Bras J M, Simon D, Pau B

机构信息

Centre de Recherches Clin-Midy, Groupe Sanofi, Montpellier, France.

出版信息

Hybridoma. 1987 Feb;6(1):97-101. doi: 10.1089/hyb.1987.6.97.

DOI:10.1089/hyb.1987.6.97

PMID:3679256

Abstract

Spleen cells of Biozzi HL mouse (selection V) immunized with bovine albumin-triiodothyronine conjugate were fused with P3-X63-Ag8.653 mouse myeloma cells. Thirteen monoclonal antibodies, selected by an enzyme-linked immunosorbent assay and a radioimmunoassay, were produced in mouse ascites fluid, purified and analyzed. All of them were IgG1 (kappa). The cross-reactivity of all these monoclonal antibodies with thyroxine (T4) was less than 0.2%. The association constant determined by Scatchard analysis ranged from 1.5 x 10(9) M-1 to 2.7 x 10(10) M-1.

摘要

用牛白蛋白 - 三碘甲状腺原氨酸偶联物免疫的Biozzi HL小鼠（选择V）的脾细胞与P3 - X63 - Ag8.653小鼠骨髓瘤细胞融合。通过酶联免疫吸附测定和放射免疫测定筛选出13种单克隆抗体，在小鼠腹水中产生，纯化并进行分析。它们均为IgG1（κ）。所有这些单克隆抗体与甲状腺素（T4）的交叉反应性均小于0.2％。通过Scatchard分析测定的缔合常数范围为1.5×10⁹ M⁻¹至2.7×10¹⁰ M⁻¹。