Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11231, Egypt; Department of Biochemistry, Faculty of Pharmacy, Heliopolis University, Cairo 11785, Egypt.
Pathol Res Pract. 2023 Jul;247:154567. doi: 10.1016/j.prp.2023.154567. Epub 2023 May 24.
Colorectal cancer (CRC) is a common tumor worldwide. CRC is influenced by several types of miRNAs and long non-coding RNAs. This study aims to evaluate the correlation of lncRNA ZFAS1/ miR200b/ ZEB1 protein with presence of CRC.
Quantitative real-time polymerase chain reaction was used to measure serum expression of lncRNA ZFAS1 and microRNA-200b in 60 CRC patients and 28 control subjects. ZEB1 protein in serum was measured by ELISA.
Lnc ZFAS1 and ZEB1 were up-regulated in CRC patients in compare to control subjects while miR-200b was down-regulated. There was a linear correlation between ZAFS1 expression and miR-200b and ZEB1 in CRC.
ZFAS1 is a key player of CRC progression and could be a potential therapeutic target by sponging miR-200b. In-addition the association between ZFAS1, miR-200b and ZEB1 highlights their potential value as a novel diagnostic biomarker in human CRC.
结直肠癌（CRC）是一种常见的肿瘤。CRC 受多种类型的 miRNA 和长非编码 RNA 的影响。本研究旨在评估 lncRNA ZFAS1/miR200b/ZEB1 蛋白与 CRC 存在的相关性。
使用实时定量聚合酶链反应测量 60 例 CRC 患者和 28 例对照者血清中 lncRNA ZFAS1 和 microRNA-200b 的表达。通过 ELISA 测量血清中 ZEB1 蛋白。
与对照组相比，CRC 患者的 lnc ZFAS1 和 ZEB1 上调，而 miR-200b 下调。在 CRC 中，ZFAS1 表达与 miR-200b 和 ZEB1 之间存在线性相关性。
ZFAS1 是 CRC 进展的关键因素，通过海绵 miR-200b 可能成为潜在的治疗靶点。此外，ZFAS1、miR-200b 和 ZEB1 之间的关联突出了它们作为人类 CRC 新型诊断生物标志物的潜在价值。
