Dhawal Pranjali P, Gharpure Milind, Joshi Minal S, Khan Rummana R, Barve Sidhivinayak S
Animal Biotechnology and Biochemistry Division, Kelkar Education Trust's Scientific Research Centre, V. G. Vaze College Campus, Mithagar Road, Mulund East, Mumbai, Maharashtra, 400081, India.
Thinq Pharma Cro Ltd A30, Rd Number 10, Wagle Estate, MIDC, Thane West, Thane, Maharashtra 400604, India.
J Ayurveda Integr Med. 2023 May-Jun;14(3):100721. doi: 10.1016/j.jaim.2023.100721. Epub 2023 May 26.
Traditional medicine in form of decoctions has been known for ages to possess wound healing abilities. One such traditional formulation mentioned in Indian literature Charak Samhita Chikitsa Sthanam is Kampillakadi Taila and tremendous information is available on its implication in the treatment of skin cuts and wounds, diseases, or bacterial infections. This research paper focuses on studying the wound healing property of one such herbal proprietary formulation known as a wound healing oil, derived from Kampillakadi Taila fortified with root extract of Wagatea spicata (VIKHPF).
The current research is aimed at studying chemical profiling, antioxidant activity, antimicrobial efficacy, in-vitro cell proliferating, and in-vitro wound healing activity of this VKHPF.
The chemical characterization of VKHPF was done by gas chromatography- fatty acid methyl esters GC-FAME analysis for lipid analysis and gas chromatography high-resolution mass spectrometry (GC-HRMS)for revealing its chemical constituents. Proliferation and migration are two underlying mechanisms involved in the healing of wounds. Hence, in-vitro studies such as cell proliferation assay and in-vitro scratch test on NIH/3T3 mice fibroblast cell line were conducted were used to determine in-vitro wound healing capacity of VKHPF. The oil was also tested for antioxidant effect (DPPH assay) and anti-microbial potential (Time kill test).
The GC-HRMS and GC-FAME analyses revealed rich medicinally important fatty acids and vitamins were present in VKHPF, such as oleic acid, hexadecanoic acid, squalene, α, γ-tocopherol, γ-sitosterol, and benzoic acid. VKHPF at 0.5 mg/ml in media without serum showed 164.00 ± 0.011% cell viability with 64.00% cell proliferation in contrast to media containing serum (100% cell viability). At the same concentration, the wound closure was 98% for VKHPF. The oil sample possessed antioxidant activity with an IC value of 3.5 mg/ml and antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa when tested using Time Kill Activity.
This study is the first to report the use of Vakeri fortified Kampillakadi Taila herbal proprietary formulation (VKHPF) in in-vitro wound healing and the present data suggest that it can form a part of modern medicine.
汤剂形式的传统医学自古以来就被认为具有伤口愈合能力。印度文献《恰拉卡本集·治疗篇》中提到的一种这样的传统配方是坎皮拉卡迪油，关于其在治疗皮肤割伤和伤口、疾病或细菌感染方面的应用有大量信息。本研究论文聚焦于研究一种源自坎皮拉卡迪油并添加了穗花瓦盖草（VIKHPF）根提取物的草药专利配方——伤口愈合油的伤口愈合特性。
当前研究旨在研究这种VIKHPF的化学剖析、抗氧化活性、抗菌功效、体外细胞增殖及体外伤口愈合活性。
通过气相色谱 - 脂肪酸甲酯（GC - FAME）分析进行脂质分析以及气相色谱高分辨率质谱（GC - HRMS）揭示其化学成分，从而对VIKHPF进行化学表征。增殖和迁移是伤口愈合涉及的两个潜在机制。因此，进行了体外研究，如对NIH/3T3小鼠成纤维细胞系进行细胞增殖测定和体外划痕试验，以确定VIKHPF的体外伤口愈合能力。还对该油进行了抗氧化作用（DPPH测定）和抗菌潜力（时间杀菌试验）测试。
GC - HRMS和GC - FAME分析表明，VIKHPF中存在丰富的具有重要药用价值的脂肪酸和维生素，如油酸、十六烷酸、角鲨烯、α、γ - 生育酚、γ - 谷甾醇和苯甲酸。在无血清培养基中，0.5mg/ml的VIKHPF显示细胞活力为164.00±0.011%，细胞增殖率为64.00%，而含血清培养基中的细胞活力为100%。在相同浓度下，VIKHPF的伤口闭合率为98%。该油样具有抗氧化活性，IC值为3.5mg/ml，并在使用时间杀菌活性测试时对金黄色葡萄球菌和铜绿假单胞菌具有抗菌活性。
本研究首次报道了添加瓦凯里的坎皮拉卡迪草药专利配方（VIKHPF）在体外伤口愈合中的应用，目前的数据表明它可以成为现代医学的一部分。
