Herbal Research Lab, Ramnarain Ruia College, Matunga (East), Mumbai 400019, India.
J Ethnopharmacol. 2011 Oct 31;138(1):99-104. doi: 10.1016/j.jep.2011.08.050. Epub 2011 Aug 30.
In traditional Indian medicinal treatise there are several Ayurvedic formulations mentioned which have been claimed as potential wound healing agents like Madhu Ghrita and Jatyadi Taila. Jatyadi Taila (JT) is a medicated oil formulation (Taila) popularly used in the treatment of various topical wounds.
Though JT has its composition recorded in ancient Ayurvedic texts, there have been minimal attempts to standardize its use in the management of wound. The current work evaluates the wound healing efficacy of JT and also provides evidence of the dermal absorption kinetics of Karanjin from JT.
JT was subjected to preliminary phytochemical evaluation. Therapeutically active marker components β-sitosterol, lupeol and karanjin were detected and separated using HPTLC. As a part of safety evaluation, skin irritation potential of JT was evaluated on rabbit skin. Excision wound model in rats were used to evaluate the wound healing efficacy of JT. Histopathological and biochemical evaluations of excised skin tissues at wound sites were carried out. The HPTLC method developed was also validated to evaluate the pharmacokinetics of Karanjin from JT after topical application on pinna of rabbit.
Preliminary phytochemical evaluation of JT revealed presence of flavonoids, essential oils, tannins, glycosides, steroids and alkaloids while resins were found to be absent. HPTLC confirmed the presence of karanjin, lupeol and β-sitosterol in JT. JT was found to be non-irritant when applied to the skin of rabbits. Topical application of JT on excision wounds caused significantly faster reduction in wound area as compared to the application of modern topical formulation (Neosporin(®)) and untreated control wounds. Animals treated with JT showed significant increase in protein, hydroxyproline and hexosamine content in the granulation tissue when compared with the untreated controls. Wound healing potential of JT was found to be dose dependant. HPTLC method was successfully used to evaluate the pharmacokinetics of Karanjin after topical application of JT on rabbit pinna.
Current work demonstrates a modern approach towards standardization of the use of traditional topical formulation JT. The results justify the traditional claim of JT for its use in the management of wounds.
在传统的印度医学著作中，有几种阿育吠陀配方被提及，据称它们是有潜力的伤口愈合剂，如 Madhu Ghrita 和 Jatyadi Taila。Jatyadi Taila（JT）是一种药用油配方（Taila），在治疗各种局部伤口方面很受欢迎。
尽管 JT 的成分在古阿育吠陀文献中有记载，但在规范其在伤口管理中的应用方面，几乎没有尝试。目前的工作评估了 JT 的伤口愈合功效，并提供了 JT 中角鲨烯的真皮吸收动力学证据。
JT 进行了初步的植物化学评估。使用 HPTLC 检测和分离治疗活性标记成分β-谷甾醇、羽扇豆醇和角鲨烯。作为安全性评估的一部分，评估了 JT 对兔皮的皮肤刺激性潜力。在大鼠的切除伤口模型中评估了 JT 的伤口愈合功效。对伤口部位切除皮肤组织进行组织病理学和生化评估。还开发了 HPTLC 方法来评估 JT 经皮给药后角鲨烯的药代动力学。
JT 的初步植物化学评估显示存在黄酮类、精油、单宁、糖苷、类固醇和生物碱，而树脂不存在。HPTLC 证实 JT 中存在角鲨烯、羽扇豆醇和β-谷甾醇。JT 应用于兔子皮肤时被发现无刺激性。与现代局部制剂（Neosporin(®)）和未处理的对照伤口相比，JT 局部应用于切除伤口可显著更快地减少伤口面积。与未处理的对照组相比，用 JT 治疗的动物在肉芽组织中显示出蛋白质、羟脯氨酸和己糖胺含量的显著增加。JT 的伤口愈合潜力与剂量有关。HPTLC 方法成功用于评估 JT 经皮给药后角鲨烯的药代动力学。
目前的工作展示了一种标准化传统局部制剂 JT 使用的现代方法。结果证明了 JT 用于治疗伤口的传统说法是合理的。
