非手术性牙周治疗后龈沟内抗菌肽 LL-37 与疼痛和牙周状况的正相关变化。一项初步研究。

Positive correlational shift between crevicular antimicrobial peptide LL-37, pain and periodontal status following non-surgical periodontal therapy. A pilot study.

机构信息

Area of Stomatology, Department of Nursing and Stomatology, Faculty of Health Sciences, Universidad Rey Juan Carlos (URJC), Avda. de Atenas s/n, Alcorcón, E-28922, Spain.

Area of Pharmacology, Nutrition and Bromatology, Department of Basic Health Sciences, Faculty of Health Sciences, Universidad Rey Juan Carlos (URJC), Unidad Asociada I+D+i Instituto de Química Médica (IQM) CSIC-URJC, Avda. de Atenas s/n, Alcorcón, E-28922, Spain.

出版信息

BMC Oral Health. 2023 May 28;23(1):335. doi: 10.1186/s12903-023-03023-w.

DOI:10.1186/s12903-023-03023-w

PMID:37246231

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10226254/

Abstract

BACKGROUND

Periodontitis has a high prevalence and uncertain recurrence. Unlike the pro-inflammatory cytokine profile, little is known about the anti-inflammatory cytokine and antimicrobial peptide overview following treatment. The present study aimed to evaluate if any of the antimicrobial peptide LL-37, interleukin (IL) 4, 10 and 6 together with the volume of gingival crevicular fluid (GCF) and total protein concentration in GCF could be used as correlative biomarkers for the severity in periodontitis as well as prognostic factors in the management of the disease.

METHODS

Forty-five participants were recruited and allocated to the healthy (15), Stage I-II (15) or Stage III-IV periodontitis (15) group. Along with periodontal examination, GCF samples were obtained at baseline and 4-6 weeks following scaling and root planing (SRP) for the periodontitis groups. GCF samples were analyzed by ELISA kits to quantify LL-37 and IL-4, -6 and - 10. One-way ANOVA followed by Dunnett's test was used to determine differences among the three groups at baseline. Two-way ANOVA followed by Sidak's post-hoc test was used to compare between pre- and post-SRP in the two periodontitis groups.

RESULTS

The amount of GCF volume was significantly correlated to the severity of periodontitis and decreased following SRP, particularly in the Stage III-IV group (p < 0.01). The levels of LL-37, IL-6, and pain and periodontal clinical parameters were significantly correlated to the severity of periodontitis. IL-4 and IL-10 in the periodontitis groups were significantly lower than the healthy group (p < 0.0001) and barely improved following SRP up to the level of the healthy group.

CONCLUSIONS

With the limitations of this study, crevicular LL-37 may be a candidate for a biomarker of periodontitis and the associated pain upon probing.

TRIAL REGISTRATION

The study was registered in clinical trials.gov, with number NCT04404335, dated 27/05/2020.

摘要

背景

牙周炎的患病率很高，且其复发情况并不确定。与促炎细胞因子的特征不同，人们对治疗后抗炎细胞因子和抗菌肽的概述知之甚少。本研究旨在评估抗菌肽 LL-37、白细胞介素 (IL) 4、10 和 6 以及龈沟液 (GCF) 的体积和 GCF 中的总蛋白浓度是否可用作牙周炎严重程度的相关生物标志物，以及该疾病管理的预后因素。

方法

招募了 45 名参与者，并将其分配到健康组（15 名）、牙周炎 I-II 期组（15 名）或牙周炎 III-IV 期组（15 名）。在牙周检查的同时，在牙周炎组接受牙周洁治和根面平整术（SRP）前后 4-6 周，获取龈沟液样本。通过 ELISA 试剂盒分析 GCF 样本，以定量测定 LL-37 和 IL-4、-6 和 -10。采用单因素方差分析（One-way ANOVA），随后采用 Dunnett 检验比较三组在基线时的差异。采用双因素方差分析（Two-way ANOVA），随后采用 Sidak 事后检验比较牙周炎两组在 SRP 前后的差异。

结果

GCF 体积的量与牙周炎的严重程度显著相关，并且在 SRP 后显著减少，尤其是在牙周炎 III-IV 期组（p<0.01）。LL-37、IL-6 和疼痛以及牙周临床参数的水平与牙周炎的严重程度显著相关。牙周炎组的 IL-4 和 IL-10 水平明显低于健康组（p<0.0001），并且在 SRP 后几乎没有改善到健康组的水平。