Synthetic Biology, University of Bremen, 28359 Bremen, Germany.
Biol Chem. 2023 May 30;405(1):55-65. doi: 10.1515/hsz-2023-0175. Print 2024 Jan 29.
Understanding how neuronal networks generate complex behavior is one of the major goals of Neuroscience. Neurotransmitter and Neuromodulators are crucial for information flow between neurons and understanding their dynamics is the key to unravel their role in behavior. To understand how the brain transmits information and how brain states arise, it is essential to visualize the dynamics of neurotransmitters, neuromodulators and neurochemicals. In the last five years, an increasing number of single-wavelength biosensors either based on periplasmic binding proteins (PBPs) or on G-protein-coupled receptors (GPCR) have been published that are able to detect neurotransmitter release and with high spatial and temporal resolution. Here we review and discuss recent progress in the development of these sensors, their limitations and future directions.
理解神经元网络如何产生复杂行为是神经科学的主要目标之一。神经递质和神经调质对于神经元之间的信息流动至关重要，理解它们的动力学是揭示它们在行为中的作用的关键。为了理解大脑如何传递信息以及大脑状态如何产生，可视化神经递质、神经调质和神经化学物质的动力学是必不可少的。在过去的五年中，越来越多的基于周质结合蛋白 (PBPs) 或 G 蛋白偶联受体 (GPCR) 的单波长生物传感器被发表，这些传感器能够以高时空分辨率检测神经递质的释放。在这里，我们回顾和讨论了这些传感器的最新进展、它们的局限性和未来的方向。
