School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Agric Food Chem. 2023 Jun 7;71(22):8508-8515. doi: 10.1021/acs.jafc.3c01288. Epub 2023 May 29.
Phenazine-1-carboxylic acid (PCA) secreted by has been commercialized and widely employed as an antifungal pesticide. However, it displays potential hazards to nontarget microorganisms and the environment. Although the PCA degradation characteristics have received extensive attention, the biodegradation efficiency is still insufficient to address the environmental risks. In this study, an engineered capable of degrading PCA was constructed by introducing heterologous PCA 1,2-dioxygenase (PcaA1A2A3A4). By integrating the PCA degradation module in the chemical mutagenesis mutant P3, 7.94 g/L PCA can be degraded in 60 h, which exhibited the highest PCA degradation efficiency to date and was 35.4-fold higher than that of the PCA natural degraders. Additionally, PCA was converted to 1-methoxyphenazine through structure modification by introducing the functional enzymes PhzS and PhzM, which has good antifungal activity and environmental compatibility. This work demonstrates new possibilities for developing PCA-derived biopesticides and enables targeted control of the impact of PCA in diverse environments.
吩嗪-1-羧酸（PCA）由 产生，已实现商业化并广泛用作抗真菌农药。然而，它对非靶标微生物和环境具有潜在危害。尽管 PCA 的降解特性受到广泛关注，但生物降解效率仍然不足以解决环境风险。在本研究中，通过引入异源 PCA 1,2-双加氧酶（PcaA1A2A3A4），构建了一种能够降解 PCA 的工程菌 。通过将 PCA 降解模块整合到化学诱变突变株 P3 中，在 60 h 内可以降解 7.94 g/L 的 PCA，这是迄今为止最高的 PCA 降解效率，比 PCA 天然降解菌高 35.4 倍。此外，通过引入功能酶 PhzS 和 PhzM 对 PCA 进行结构修饰，将其转化为 1-甲氧基吩嗪，具有良好的抗真菌活性和环境相容性。这项工作为开发 PCA 衍生的生物农药提供了新的可能性，并能够针对 PCA 在不同环境中的影响进行靶向控制。
