Key Laboratory of Entomology and Pest Control Engineering, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing 400715, China.
Key Laboratory of Entomology and Pest Control Engineering, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing 400715, China.
Pestic Biochem Physiol. 2023 Jun;193:105443. doi: 10.1016/j.pestbp.2023.105443. Epub 2023 Apr 26.
Carboxylesterases (CarEs) are a multifunctional superfamily of enzymes and play an important role in detoxification of various insecticides in insects. The oriental fruit fly, Bactrocera dorsalis, is one of the most destructive agricultural pests and has developed different degrees of resistance to organophosphates in field. However, the involvement of BdCarEs in tolerance or resistance to other alternative insecticides are still unclear. In the present study, 33 BdCarEs genes were identified based on the genome database of B. dorsalis. Phylogenetic analysis demonstrated that they were classified into nine clades, with abundance of α-esterases. Meanwhile, the sequence characterization and the chromosome distribution were also analyzed. The spatiotemporal expression analysis of BdCarEs genes suggested that the diversity of potential function in different physiological processes. With the exception of BdCarE21, all BdCarEs genes responded to at least one insecticide exposure, and BdCarE20 was found to be up-regulated after exposure to all five tested insecticides individually. Eight BdCarEs genes were overexpressed in MR strain when compared to that in SS strain. Subsequently, knockdown the expression of representative BdCarEs genes significantly increased the susceptibility of the oriental fruit fly to corresponding insecticides, which indicated that the tested BdCarEs genes contributed to one or multiple insecticide detoxification. These findings provide valuable insights into the potential role in respond to tolerance or resistance to insecticides with different mode of action, and will facilitate development of efficiency management strategy for B. dorsalis.
羧酸酯酶（CarEs）是一个多功能的超家族酶，在昆虫对各种杀虫剂的解毒中起着重要作用。东方果实蝇，Bactrocera dorsalis，是一种极具破坏性的农业害虫，在田间对有机磷农药产生了不同程度的抗性。然而，BdCarEs 参与对其他替代杀虫剂的耐受或抗性的情况仍不清楚。在本研究中，基于 B. dorsalis 的基因组数据库鉴定了 33 个 BdCarEs 基因。系统发育分析表明，它们分为 9 个分支，富含 α-酯酶。同时，还分析了序列特征和染色体分布。BdCarEs 基因的时空表达分析表明，它们在不同生理过程中具有潜在功能的多样性。除了 BdCarE21 之外，所有 BdCarEs 基因都至少对一种杀虫剂暴露有反应，并且发现 BdCarE20 在单独暴露于五种测试的杀虫剂后上调。与 SS 品系相比，MR 品系中有 8 个 BdCarEs 基因过度表达。随后，敲低代表性 BdCarEs 基因的表达显著增加了东方果实蝇对相应杀虫剂的敏感性，这表明测试的 BdCarEs 基因参与了一种或多种杀虫剂的解毒。这些发现为不同作用模式的杀虫剂耐受或抗性的响应提供了有价值的见解，并将有助于开发针对 B. dorsalis 的高效管理策略。
