Lee Gyeongmin, Kang Hyo Rin, Kim Aro, Park Jong-Hyeon, Lee Min Ja, Kim Su-Mi
Center for Foot-and-Mouth Disease Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
Front Microbiol. 2023 May 12;14:1121830. doi: 10.3389/fmicb.2023.1121830. eCollection 2023.
Foot-and-mouth disease (FMD) is an acute contagious infectious disease that affects cloven-hoofed animals. Although current emergency FMD vaccines only take effect 7 days after vaccination, antiviral agents, such as quercetin, which is a common flavonoid, could reduce the spread of FMD virus (FMDV) during outbreaks. We investigated the and antiviral effects of quercetin against FMDV. Analysis of viral copy numbers showed that quercetin had a dose-dependent inhibitory effect on FMDV at concentrations between 19.5 and 1,250 μM in porcine cells. In addition, we observed a quercetin-induced interferon (IFN)-α protein and interferon-stimulated gene (ISG) upregulation in swine cells. Enzyme-linked immunosorbent assay of sera revealed that quercetin induces the production of IFN-α, IFN-β, IFN-γ, interleukin (IL)-12, and IL-15 in mice. Inoculation of mice with quercetin or a combination of quercetin with an inactivated FMD vaccine enhanced both the survival rate and neutralizing antibody titer. Therefore, we suggest the use of quercetin as a novel and effective antiviral agent for controlling FMDV infection; however, further investigation of its application in livestock is required.
口蹄疫（FMD）是一种影响偶蹄类动物的急性传染性疾病。尽管目前的口蹄疫紧急疫苗在接种后7天才会生效，但抗病毒药物，如常见的类黄酮槲皮素，在疫情爆发期间可以减少口蹄疫病毒（FMDV）的传播。我们研究了槲皮素对FMDV的抗病毒作用。病毒拷贝数分析表明，在猪细胞中，浓度介于19.5至1250μM之间的槲皮素对FMDV具有剂量依赖性抑制作用。此外，我们观察到在猪细胞中槲皮素诱导干扰素（IFN）-α蛋白和干扰素刺激基因（ISG）上调。血清酶联免疫吸附测定显示，槲皮素可诱导小鼠产生IFN-α、IFN-β、IFN-γ、白细胞介素（IL）-12和IL-15。用槲皮素或槲皮素与灭活口蹄疫疫苗的组合接种小鼠，可提高存活率和中和抗体滴度。因此，我们建议将槲皮素用作控制FMDV感染的新型有效抗病毒药物；然而，需要进一步研究其在牲畜中的应用。
