Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, 510000, China.
BMC Vet Res. 2024 Apr 3;20(1):134. doi: 10.1186/s12917-024-03984-2.
Porcine acute diarrhea syndrome coronavirus (SADS-CoV) is one of the novel pathogens responsible for piglet diarrhea, contributing to substantial economic losses in the farming sector. The broad host range of SADS-CoV raises concerns regarding its potential for cross-species transmission. Currently, there are no effective means of preventing or treating SADS-CoV infection, underscoring the urgent need for identifying efficient antiviral drugs. This study focuses on evaluating quercetin as an antiviral agent against SADS-CoV.
In vitro experiments showed that quercetin inhibited SADS-CoV proliferation in a concentration-dependent manner, targeting the adsorption and replication stages of the viral life cycle. Furthermore, quercetin disrupts the regulation of the P53 gene by the virus and inhibits host cell cycle progression induced by SADS-CoV infection. In vivo experiments revealed that quercetin effectively alleviated the clinical symptoms and intestinal pathological damage caused by SADS-CoV-infected piglets, leading to reduced expression levels of inflammatory factors such as TLR3, IL-6, IL-8, and TNF-α.
Therefore, this study provides compelling evidence that quercetin has great potential and promising applications for anti- SADS-CoV action.
猪急性腹泻综合征冠状病毒（SADS-CoV）是一种新型病原体，可导致仔猪腹泻，给农业部门造成重大经济损失。SADS-CoV 的广泛宿主范围引起了人们对其跨物种传播潜力的关注。目前，尚无预防或治疗 SADS-CoV 感染的有效方法，这突显了迫切需要寻找有效的抗病毒药物。本研究聚焦于评估槲皮素作为一种抗 SADS-CoV 的抗病毒药物。
体外实验表明，槲皮素呈浓度依赖性抑制 SADS-CoV 的增殖，靶向病毒生命周期的吸附和复制阶段。此外，槲皮素破坏了病毒对 P53 基因的调控，并抑制了 SADS-CoV 感染诱导的宿主细胞周期进程。体内实验表明，槲皮素可有效缓解 SADS-CoV 感染仔猪的临床症状和肠道病理损伤，降低 TLR3、IL-6、IL-8 和 TNF-α 等炎症因子的表达水平。
因此，本研究提供了有力的证据表明，槲皮素有很大的潜力和应用前景，可用于抗 SADS-CoV 作用。
