Wu Bo, Xiu Jianlong, Yu Li, Huang Lixin, Yi Lina, Ma Yuandong
School of Engineering Science, University of Chinese Academy of Sciences, Beijing, China.
Institute of Porous Flow and Fluid Mechanics, University of Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2023 May 12;14:1132831. doi: 10.3389/fmicb.2023.1132831. eCollection 2023.
Microbial remediation has been regarded as one of the most promising decontamination techniques for crude oil pollution. However, there are few studies on the interaction of bacteria in the microbial community during bioremediation. The aim of this work was to research the promotion of defined co-culture of SL and WJ-1 for biodegradation of crude oil. After 7 days of incubation, the analysis of residual oil, saturated and aromatic fraction in the samples showed that the degradation efficiency of them was significantly improved. The degradation efficiency of crude oil was enhanced from 32.61% and 54.35% in individual culture to 63.05% by the defined co-culture of strains SL and WJ-1. Furthermore, it was found that the defined co-culture system represented relatively excellent performance in bacterial growth, cell surface hydrophobicity (CSH) and emulsification activity. These results indicated that the combination of and can effectively promote the degradation and utilization of crude oil, which may provide a new idea for the improvement of bioremediation strategies. GRAPHICAL ABSTRACT.
微生物修复被认为是原油污染最具前景的去污技术之一。然而，关于生物修复过程中微生物群落中细菌相互作用的研究很少。这项工作的目的是研究特定的SL和WJ-1共培养对原油生物降解的促进作用。培养7天后，对样品中残留油、饱和馏分和芳烃馏分的分析表明，它们的降解效率显著提高。原油降解效率从单独培养时的32.61%和54.35%通过菌株SL和WJ-1的特定共培养提高到63.05%。此外，发现特定共培养系统在细菌生长、细胞表面疏水性（CSH）和乳化活性方面表现出相对优异的性能。这些结果表明，SL和WJ-1的组合可以有效促进原油的降解和利用，这可能为改进生物修复策略提供新思路。图形摘要。
