Locatelli Clara, Gattolin Stefano, Monistero Valentina, Castiglioni Bianca, Moroni Paolo, Addis Maria Filippa, Cremonesi Paola
Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Lodi, Italy.
Laboratorio di Malattie Infettive degli Animali, Università degli Studi di Milano, Lodi, Italy.
Front Microbiol. 2023 May 11;14:1120305. doi: 10.3389/fmicb.2023.1120305. eCollection 2023.
Accurate and precise differentiation of staphylococci isolated from milk is of importance for udder health management. In particular, the rapid and specific identification of plays an essential role in the prevention and treatment programs for bovine mastitis. Plasma gelatinization in coagulase assays is routinely used to discriminate from other species by detecting the presence of extracellular free staphylocoagulase. However, rarely occurring coagulase-deficient strains can be responsible for clinical and subclinical mastitis cases. By investigating isolates from a single herd over a 10-year period we identified the persistence of a phenotypically coagulase-negative strain and pinpointed the possible cause to a single base pair deletion in the gene sequence. Our results support the need to integrate primary biochemical tests with molecular/sequence analysis approaches for correctly identifying and discriminating atypical in bovine herds, as the coagulase test alone may fail to detect persistent mastitis-causing strains.
准确且精确地区分从牛奶中分离出的葡萄球菌对于乳房健康管理至关重要。特别是，快速且特异性地鉴定[具体内容缺失]在奶牛乳腺炎的预防和治疗方案中起着至关重要的作用。凝固酶试验中的血浆凝胶化通常用于通过检测细胞外游离葡萄球菌凝固酶的存在来区分[具体内容缺失]与其他物种。然而，罕见的凝固酶缺陷型[具体内容缺失]菌株可能导致临床和亚临床乳腺炎病例。通过对一个牛群10年间分离出的[具体内容缺失]进行调查，我们确定了一种表型凝固酶阴性的[具体内容缺失]菌株的持续性，并将可能的原因定位到[具体内容缺失]基因序列中的一个单碱基对缺失。我们的结果支持需要将初步生化试验与分子/序列分析方法相结合，以正确识别和区分牛群中的非典型[具体内容缺失]，因为仅靠凝固酶试验可能无法检测出导致持续性乳腺炎的菌株。
