Saraiva Mauro M S, Rodrigues Heitor Leocádio de Souza, Benevides Valdinete Pereira, de Leon Candice Maria Cardoso Gomes, Santos Silvana C L, Stipp Danilo T, Givisiez Patricia E N, Vieira Rafael F C, Oliveira Celso J B
Department of Animal Science, College for Agricultural Sciences, Federal University of Paraiba (CCA/UFPB), Areia 58397-000, PB, Brazil.
School of Agricultural and Veterinary Sciences (FCAV/Unesp), São Paulo State University, Jaboticabal 14885-900, SP, Brazil.
Antibiotics (Basel). 2025 Jul 9;14(7):693. doi: 10.3390/antibiotics14070693.
() is a major cause of opportunistic infections in humans and animals, leading to severe systemic diseases. The rise of MDR strains associated with animal carriage poses significant health challenges, underscoring the need to investigate animal-derived .
This study examined the genotypic relatedness and phenotypic profiles of antimicrobial resistance in . , previously sampled from nostril swabs of healthy horses from two geographically distant Brazilian states (Northeast and South), separated by over 3700 km. The study also sought to confirm the presence of methicillin-resistant (MRSA) and borderline oxacillin-resistant (BORSA) strains and to characterize the isolates through molecular typing using PCR.
Among 123 screened staphylococci, 21 isolates were confirmed as via biochemical tests and PCR targeting species-specific genes (, , ).
REP-PCR analysis generated genotypic profiles, revealing four antimicrobial resistance patterns, with MDR observed in ten isolates. Six isolates exhibited cefoxitin resistance, suggesting methicillin resistance, despite the absence of the gene. REP-PCR demonstrated high discriminatory power, grouping the isolates into five major clusters.
The genotyping indicated no clustering by geographical origin, highlighting significant genetic diversity among strains colonizing horses' nostrils in Brazil. These findings highlight the widespread and varied nature of among horses, contributing to a deeper understanding of its epidemiology and resistance profiles in animals across diverse regions. Ultimately, this genetic diversity can pose a public health risk that the epidemiological surveillance services must investigate.
（某病菌名称）是人类和动物机会性感染的主要原因，可导致严重的全身性疾病。与动物携带相关的多重耐药菌株的出现带来了重大的健康挑战，凸显了对动物源（该病菌名称）进行调查的必要性。
本研究检查了（该病菌名称）的基因型相关性和抗菌药物耐药性的表型特征。这些菌株先前从巴西两个地理上相距遥远的州（东北部和南部）健康马匹的鼻孔拭子中采集，两地相距超过3700公里。该研究还试图确认耐甲氧西林金黄色葡萄球菌（MRSA）和临界耐苯唑西林金黄色葡萄球菌（BORSA）菌株的存在，并通过使用PCR的分子分型来鉴定分离株。
在123株筛选出的葡萄球菌中，通过生化试验和针对种特异性基因（具体基因名称）的PCR确认了21株为（该病菌名称）。
REP-PCR分析产生了基因型图谱，揭示了四种抗菌药物耐药模式，其中10株分离株表现出多重耐药性。6株分离株表现出头孢西丁耐药性，表明存在耐甲氧西林性，尽管不存在（相关基因名称）基因。REP-PCR显示出高鉴别力，将分离株分为五个主要簇。
基因分型表明没有按地理来源聚类，突出了巴西马匹鼻孔中定植的（该病菌名称）菌株之间存在显著的遗传多样性。这些发现突出了（该病菌名称）在马匹中广泛且多样的性质，有助于更深入地了解其在不同地区动物中的流行病学和耐药谱。最终，这种遗传多样性可能构成公共卫生风险，流行病学监测服务必须对此进行调查。
