Wang Xi, Wu Yifan, Liu Yanli, Chen Feihong, Chen Sijie, Zhang Feiyu, Li Shujin, Wang Chaowei, Gong Yi, Huang Ruitian, Hu Minhan, Ning Yujie, Zhao Hongmou, Guo Xiong
Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.
Key Laboratory of Trace Elements and Endemic Diseases, School of Public Health, Xi'an Jiaotong University Health Science Center, National Health and Family Planning Commission, Xi'an, Shaanxi, China.
Front Microbiol. 2023 May 12;14:1153424. doi: 10.3389/fmicb.2023.1153424. eCollection 2023.
Osteoarthritis (OA) is a kind of chronic, degenerative disorder with unknown causes. In this study, we aimed to improve our understanding of the gut microbiota profile in patients with knee OA.
16S rDNA gene sequencing was performed to detect the gut microbiota in fecal samples collected from the patients with OA ( = 32) and normal control (NC, = 57). Then the metagenomic sequencing was used to identify the genes or functions linked with gut microbial changes at the species level in the fecal samples from patients with OA and NC groups.
The Proteobacteria was identified as dominant bacteria in OA group. We identified 81 genera resulted significantly different in abundance between OA and NC. The abundance of , , , , and showed significant decrease in the OA compared to the NC. The abundance of genera , , and were increasing in the OA group, and the families , , and were increasing in the NC. The metagenomic sequencing showed that the abundance of , and at the species level were significantly decreasing in the OA, and the abundance of , , and were significantly increased in OA.
The results of our study interpret a comprehensive profile of the gut microbiota in patients with knee OA and offer the evidence that the cartilage-gut-microbiome axis could play a crucial role in underlying the mechanisms and pathogenesis of OA.
骨关节炎（OA）是一种病因不明的慢性退行性疾病。在本研究中，我们旨在加深对膝骨关节炎患者肠道微生物群特征的理解。
对从骨关节炎患者（n = 32）和正常对照组（NC，n = 57）收集的粪便样本进行16S rDNA基因测序，以检测肠道微生物群。然后，使用宏基因组测序来鉴定与骨关节炎患者和NC组粪便样本中物种水平上肠道微生物变化相关的基因或功能。
变形菌门被确定为骨关节炎组中的优势菌。我们鉴定出81个属在骨关节炎组和NC组之间的丰度存在显著差异。与NC组相比，骨关节炎组中[具体菌属名称1]、[具体菌属名称2]、[具体菌属名称3]、[具体菌属名称4]和[具体菌属名称5]的丰度显著降低。骨关节炎组中[具体菌属名称6]、[具体菌属名称7]、[具体菌属名称8]和[具体菌属名称9]的属丰度增加，而NC组中[具体菌科名称1]、[具体菌科名称2]、[具体菌科名称3]和[具体菌科名称4]的科丰度增加。宏基因组测序显示，骨关节炎组中物种水平上[具体物种名称1]、[具体物种名称2]和[具体物种名称3]的丰度显著降低，而骨关节炎组中[具体物种名称4] [具体物种名称5]、[具体物种名称6]和[具体物种名称7]的丰度显著增加。
我们的研究结果解释了膝骨关节炎患者肠道微生物群的综合特征，并提供了证据表明软骨-肠道-微生物群轴可能在骨关节炎的机制和发病过程中起关键作用。
