Liu Jinqiao, Tang Jianpu, Tong Zhaobin, Teng Guangshuai, Yang Dayong
Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), Institute of Biomolecular and Biomedical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P.R. China.
Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China.
iScience. 2023 Apr 8;26(5):106620. doi: 10.1016/j.isci.2023.106620. eCollection 2023 May 19.
Self-assembly processes exist widely in life systems and play essential roles in maintaining life activities. It is promising to explore the molecular fundamentals and mechanisms of life systems through artificially constructing self-assembly systems in living cells. As an excellent self-assembly construction material, deoxyribonucleic acid (DNA) has been widely used to achieve the precise construction of self-assembly systems in living cells. This review focuses on the recent progress of DNA-guided intracellular self-assembly. First, the methods of intracellular DNA self-assembly based on the conformational transition of DNA are summarized, including complementary base pairing, the formation of G-quadruplex/i-motif, and the specific recognition of DNA aptamer. Next, The applications of DNA-guided intracellular self-assembly on the detection of intracellular biomolecules and the regulation of cell behaviors are introduced, and the molecular design of DNA in the self-assembly systems is discussed in detail. Ultimately, the challenges and opportunities of DNA-guided intracellular self-assembly are commented.
自组装过程广泛存在于生命系统中，并在维持生命活动中发挥着至关重要的作用。通过在活细胞中人工构建自组装系统来探索生命系统的分子基础和机制是很有前景的。作为一种优秀的自组装构建材料，脱氧核糖核酸（DNA）已被广泛用于在活细胞中实现自组装系统的精确构建。本综述重点关注DNA引导的细胞内自组装的最新进展。首先，总结了基于DNA构象转变的细胞内DNA自组装方法，包括互补碱基配对、G-四链体/i-基序的形成以及DNA适配体的特异性识别。接下来，介绍了DNA引导的细胞内自组装在细胞内生物分子检测和细胞行为调控方面的应用，并详细讨论了自组装系统中DNA的分子设计。最后，对DNA引导的细胞内自组装的挑战和机遇进行了评述。
