Link.的挥发性成分概况及微繁殖条件
Volatile profile and micropropagation conditions of Link.
作者信息
Pinheiro Marcos Vinícius Marques, Silva Maria Luara Aragão, da Silva Karina Vieira, de Paula Alves Juliana, Dos Santos Marinho Tácila Rayene, Alves Givago Lopes, Catunda Junior Francisco Eduardo Aragão, Dos Santos Monteiro Odair, de Assis Figueiredo Fábio Afonso Mazzei Moura, Corrêa Thais Roseli, Batista Diego Silva
机构信息
Programa de Pós-Graduação Em Agricultura E Ambiente, Universidade Estadual Do Maranhão, São Luís, MA 65055-310 Brazil.
Programa de Pós-Graduação Em Agroecologia, Universidade Estadual Do Maranhão, São Luís, MA 65055-310 Brazil.
出版信息
3 Biotech. 2023 Jun;13(6):212. doi: 10.1007/s13205-023-03634-8. Epub 2023 May 25.
UNLABELLED
Link. is a native South American plant, which possesses volatile compounds with pharmaceutical and medicinal properties such as antidiabetic and anti-inflammatory effects. However, the conservation and propagation of this plant are complicated by its recalcitrant seeds and delayed flowering transition. Hence, tissue culture is employed for the safe and efficient propagation of . However, the optimal conditions for the in vitro cultivation of remain unknown. Thus, this study aimed to characterize the volatile profile of adult field plants and evaluate the effects of different light intensities (43 and 70 μmol m s), gas exchange rates (14 and 25 µL L s), and exogenous sucrose concentrations (0, 20, and 30 g L) on their in vitro development. The results showed that β-caryophyllene is the major volatile compound produced by . Moreover, culturing in a medium containing 30 g L of sucrose and flasks with membranes that allow CO exchange at the rate of 25 µL L s produced vigorous and hardened plants with high survival rates independent of irradiance. This study is the first to report the optimal in vitro culture conditions for as a reference for future studies on micropropagation and secondary metabolite production using this species.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-023-03634-8.
未标记
Link.是一种原产于南美洲的植物，它含有具有药用和治疗特性的挥发性化合物，如抗糖尿病和抗炎作用。然而，这种植物的种子顽拗性和开花过渡延迟使其保护和繁殖变得复杂。因此，采用组织培养来安全有效地繁殖Link.。然而，Link.体外培养的最佳条件仍然未知。因此，本研究旨在表征成年Link.田间植株的挥发性成分，并评估不同光照强度（43和70 μmol m² s⁻¹）、气体交换速率（14和25 μL L⁻¹ s⁻¹）和外源蔗糖浓度（0、20和30 g L⁻¹）对其体外发育的影响。结果表明，β-石竹烯是Link.产生的主要挥发性化合物。此外，在含有30 g L⁻¹蔗糖的培养基中培养，并使用允许以25 μL L⁻¹ s⁻¹的速率进行CO₂交换的膜的培养瓶，可产生健壮且硬化的植株，其存活率高，且与光照无关。本研究首次报道了Link.的最佳体外培养条件，可为今后利用该物种进行微繁殖和次生代谢产物生产的研究提供参考。
补充信息
在线版本包含可在10.1007/s13205-023-03634-8获取的补充材料。
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