Lin Hai, Zhang Liyan, Liu Bin, Cui Guopeng
Department of Spine Surgery, The Sixth Affiliated Hospital of Wenzhou Medical University (The People's Hospital of LiShui), Zhejiang, 323000, China.
Curr Cancer Drug Targets. 2023 May 29. doi: 10.2174/1568009623666230529140739.
Ubiquitin ligases (E3s) play an important role in multiple cancers.
The open-accessed expression profile and clinical information was downloaded from the TARGET database. The analysis was performed using R software.
In this study, we comprehensively investigated the role of E3s in osteosarcomas (OS). We found that among all these E3s, UBR5 is a risk factor for OS. Considering that UBR5 has not been reported in previous studies focused on OS, we selected it for further analysis. Interestingly, we found that UBR5 had no significant effect on immune cell infiltration but a remarkable effect on immune function. Moreover, we divided the patients into "immune activation" and "immune exhaustion" types. KM survival curves indicated that the patients in the "immune exhaustion" types had a worse survival performance. Further, we identified the molecules involved in immune function and significantly correlated with UBR5. The biological enrichment analysis and prognosis model were then conducted based on these genes. Results indicated that the patients in the high-risk group had a worse survival performance, and underlying biological differences between high and low-risk patients were also explored. Ultimately, the effect pattern of UBR5 in pan-cancer was also explored.
In summary, our study comprehensively explored the role of UBR5 in OS, as well as its effect on the immune microenvironment, which might be an underlying therapy target.
泛素连接酶（E3s）在多种癌症中发挥重要作用。
从TARGET数据库下载公开获取的表达谱和临床信息。使用R软件进行分析。
在本研究中，我们全面研究了E3s在骨肉瘤（OS）中的作用。我们发现，在所有这些E3s中，UBR5是OS的一个危险因素。鉴于UBR5在先前针对OS的研究中尚未有报道，我们选择对其进行进一步分析。有趣的是，我们发现UBR5对免疫细胞浸润无显著影响，但对免疫功能有显著影响。此外，我们将患者分为“免疫激活”型和“免疫耗竭”型。KM生存曲线表明，“免疫耗竭”型患者的生存表现较差。进一步地，我们确定了参与免疫功能且与UBR5显著相关的分子。然后基于这些基因进行生物富集分析和预后模型构建。结果表明，高危组患者的生存表现较差，同时还探究了高危和低危患者之间潜在的生物学差异。最终，还探究了UBR5在泛癌中的作用模式。
总之，我们的研究全面探讨了UBR5在OS中的作用及其对免疫微环境的影响，这可能是一个潜在的治疗靶点。
