Instituto de Investigação, Inovação e Desenvolvimento (FP-I3ID), Universidade Fernando Pessoa (UFP), Escola Superior de Saúde, Praça de 9 de Abril, 349, 4249-004 Porto, Portugal.
Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Departamento de Microbiologia e Parasitologia, Rua Frei Caneca, 94, Centro, 20211-040 Rio de Janeiro, RJ, Brazil.
An Acad Bras Cienc. 2023 May 29;95(suppl 1):e20220578. doi: 10.1590/0001-3765202320220578. eCollection 2023.
The influence of refrigeration on the post-embryonic development of Chrysomya putoria larvae was evaluated, regarding its resistance in the logistics of storage and distribution in biotherapy. Previously sterilized larvae were submitted to four periods of storage under refrigeration (T1=12 h, T2=24 h, T3=48 h and T4=72 h) and control (without sterilization and refrigeration). Newly hatched larvae (0.200 g) were stored between 3 and 9ºC. After refrigeration, 40 neo-larvae (in triplicate) were transferred to 50 g of protein diet and incubated in an acclimatized chamber. There was a significant difference in the larval body mass (T1 and T2) and in the duration of larval, pupal and total development (T3 and T4). The sex ratios found in the four treatments did not differ from what was expected. Normality rates were 100% for all treatments. There was no significant difference between the Control, T1 and T2 treatments for larval, pupal and total viability. There was a significant difference between control (C) and T4 (larval viability), between C, T3 and T4 (pupa) and between C and T4 (total). C. putoria has resistance under refrigeration and storage of up to 56 h, presenting viability above 70% for use in biotherapy.
研究了冷藏对蕈蚊幼虫（Chrysomya putoria）胚胎后发育的影响，因为其在生物治疗的储存和配送物流中有一定的抵抗能力。先前已消毒的幼虫被置于冷藏（T1=12 h、T2=24 h、T3=48 h 和 T4=72 h）和对照（未经消毒和冷藏）下进行了四个时期的储存。新孵化的幼虫（0.200 g）在 3 至 9°C 之间储存。冷藏后，将 40 只新幼虫（一式三份）转移到 50 g 蛋白质饮食中，并在适应室中孵育。幼虫体质量（T1 和 T2）和幼虫、蛹和总发育时间（T3 和 T4）有显著差异。四个处理中的性别比例与预期的没有差异。所有处理的正常率均为 100%。对照、T1 和 T2 处理的幼虫、蛹和总存活率之间无显著差异。T4 处理与对照组（C）（幼虫存活率）、T3、T4 处理与对照组（C）（蛹）以及 C 与 T4 处理（总存活率）之间有显著差异。蕈蚊在冷藏和储存长达 56 小时内具有抗性，其生物治疗存活率超过 70%。
