抗生素庆大霉素对恶臭绿蝇（双翅目：丽蝇科）胚后发育的影响。
Effects of the antibiotics Gentamicin on the postembryonic development of Chrysomya putoria (Diptera: Calliphoridae).
作者信息
Ferraz Adriana C P, Dallavecchia Daniele L, Silva Débora C, Figueiredo Adriana L, Proença Barbara, Silva-Filho Renato G, Aguiar Valéria M
机构信息
Departamento de Microbiologia e Parasitologia, Laboratório de Estudo de Dípteros, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, UNIRIO, Rua Frei Caneca, 94, Centro, Cep 20211-040, Rio de Janeiro, RJ, Brasil Universidade Federal Rural do Rio de Janeiro, UFRRJ, Programa de Pós-Graduação em Biologia Animal PPGBA, Rod. BR 465, Km 7, CEP 23890-000 Seropédica, RJ, Brasil.
Departamento de Microbiologia e Parasitologia, Laboratório de Estudo de Dípteros, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, UNIRIO, Rua Frei Caneca, 94, Centro, Cep 20211-040, Rio de Janeiro, RJ, Brasil Universidade Federal do Estado do Rio de Janeiro, UNIRIO, Programa de Pós-Graduação em Ciências Biológicas PPGBIO, URCA, RJ, Avenida Pasteur, 296, CEP 22290-240, Rio de Janeiro, RJ, Brasil.
出版信息
J Insect Sci. 2014 Jan 1;14. doi: 10.1093/jisesa/ieu141. Print 2014.
We evaluate the effects the antibiotic Gentamicin on the development of Chrysomya putoria (Wiedemann, 1818). Third-generation, first-instar larvae were reared in a climatic chamber on 60 g of homogenate + agar 65% and were treated with three concentrations of Gentamicin: 4.44 mg/ml, 13.33 mg/ml, and 66.66 mg/ml. The control consisted of distilled water. The relationships between mean body mass of mature larvae (measured after diet abandonment, in batches of five individuals), duration of larval and pupal stages, and overall duration of development were analyzed. The actual sex ratio was compared against the expected using the chi square. None of the parameters measured differed significantly among the four treatments, with one exception: when Gentamicin concentration was 13.33 mg/ml, larval viability differed significantly from the control. All larvae from all treatments were considered normal. We conclude that the antibiotic did not significantly alter the development of C. putoria (Wiedemann) (Diptera: Calliphoridae).
我们评估了抗生素庆大霉素对恶臭金蝇（Chrysomya putoria (Wiedemann, 1818)）发育的影响。将第三代一龄幼虫饲养在气候箱中，以60克匀浆 + 65%琼脂为食，并分别用三种浓度的庆大霉素进行处理：4.44毫克/毫升、13.33毫克/毫升和66.66毫克/毫升。对照组为蒸馏水。分析了成熟幼虫的平均体重（在幼虫停止取食后，每组五只个体进行测量）、幼虫期和蛹期的持续时间以及发育总时长之间的关系。使用卡方检验将实际性别比与预期性别比进行比较。在四种处理中，除了一个例外，所测量的参数均无显著差异：当庆大霉素浓度为13.33毫克/毫升时，幼虫活力与对照组有显著差异。所有处理组的幼虫均被视为正常。我们得出结论，该抗生素并未显著改变恶臭金蝇（Chrysomya putoria (Wiedemann)）（双翅目：丽蝇科）的发育。
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