Chen Quan, Bao Li, Yue Zhendong, Wang Lei, Fan Zhenhua, Liu Fuquan
Department of Interventional Therapy, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
Front Mol Biosci. 2023 May 15;10:1168782. doi: 10.3389/fmolb.2023.1168782. eCollection 2023.
Transjugular intrahepatic portal shunt (TIPS) insertion could promote weight gain and muscle and fat mass increase in patients with cirrhosis. However, few studies have focused on metabolic changes after TIPS. This study aims to explore metabolic changes after TIPS and potential biomarkers of adverse events. Peripheral and portal serum samples were collected before and after TIPS insertion. Untargeted metabolomics was performed using ultra-high-performance liquid chromatography-mass spectrometry. Spearman's correlation analysis was used to determine the relationship between metabolites and clinical parameters. Metabolite set enrichment analysis was performed to explore enriched pathways. The predictive value of the metabolites was calculated by receiver operating characteristic curve (ROC) analysis. Metabolites in the peripheral and portal serum significantly changed early after TIPS. Some lipid metabolites were significantly correlated with liver function parameters. Both elevated and depleted metabolites were mainly enriched in amino acid metabolism. Nine and 12 portal metabolites have moderate predictive value in post-TIPS liver function decline and hepatic encephalopathy (HE), separately (area under curve >0.7). Metabolites in the peripheral and portal veins significantly changed after TIPS. Some metabolic changes might be ascribed to liver function decline early after TIPS. Nine and 12 portal metabolites might be potential biomarkers in prediction of liver function decline and HE, separately.
经颈静脉肝内门体分流术(TIPS)置入可促进肝硬化患者体重增加以及肌肉和脂肪量增加。然而,很少有研究关注TIPS术后的代谢变化。本研究旨在探讨TIPS术后的代谢变化及不良事件的潜在生物标志物。在TIPS置入前后采集外周血和门静脉血清样本。使用超高效液相色谱-质谱联用技术进行非靶向代谢组学分析。采用Spearman相关性分析确定代谢物与临床参数之间的关系。进行代谢物集富集分析以探索富集途径。通过受试者工作特征曲线(ROC)分析计算代谢物的预测价值。TIPS术后早期外周血和门静脉血清中的代谢物显著变化。一些脂质代谢物与肝功能参数显著相关。代谢物的升高和减少主要富集在氨基酸代谢中。分别有9种和12种门静脉代谢物对TIPS术后肝功能下降和肝性脑病(HE)具有中等预测价值(曲线下面积>0.7)。TIPS术后外周血和门静脉中的代谢物显著变化。一些代谢变化可能归因于TIPS术后早期肝功能下降。分别有9种和12种门静脉代谢物可能是预测肝功能下降和HE的潜在生物标志物。