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一种新颖的家庭用尿液样本微生物负荷鉴定培养机制。

A novel home-use culture mechanism for identifying microbial load in urine samples.

机构信息

Samplytics Technologies Pvt. Ltd., Koramangala, Bangalore, India.

出版信息

PLoS One. 2023 May 31;18(5):e0285881. doi: 10.1371/journal.pone.0285881. eCollection 2023.

Abstract

Diagnosing a urinary tract infection (UTI) is typically a clinical procedure involving multiple steps. The need to perform a test depends on the presence of relevant symptoms. Given the current pandemic situation, visiting a clinic may not be a preferable choice for many users. Many vulnerable groups of patients, namely, males with certain predispositions and pregnant women, may not present with symptoms of UTI and may go undiagnosed, which could give rise to a more complicated situation. So far, microbial cultures have been used as the gold standard for the diagnosis of an infection. However, performing microbial cultures currently requires trained professionals and laboratory grade equipment. Therefore, there is a need for a home-use culture kit that can serve this purpose. To our knowledge, no such kit exists. Here, we present a feasibility study of an affordable and easy-to-use home-based setup for quantifying bacterial load in a urine. We believe that such a system can be used by people at home to monitor recurrent UTI infections and also by physicians to remotely monitor and prescribe narrow-spectrum antibiotics for more effective treatment.

摘要

诊断尿路感染(UTI)通常是一个涉及多个步骤的临床过程。是否需要进行检测取决于是否存在相关症状。考虑到当前的大流行情况,对于许多用户来说,去诊所就诊可能不是首选。许多易感染的患者群体,即具有某些倾向的男性和孕妇,可能没有出现 UTI 症状,因此可能无法被诊断出来,这可能会导致更复杂的情况。到目前为止,微生物培养一直被用作感染诊断的金标准。然而,目前进行微生物培养需要经过培训的专业人员和实验室级别的设备。因此,需要一种可以满足这一需求的家用培养试剂盒。据我们所知,目前还没有这样的试剂盒。在这里,我们提出了一项关于经济实惠且易于使用的家用装置来定量尿液中细菌负荷的可行性研究。我们相信,这样的系统可以供人们在家中用于监测复发性 UTI 感染,也可以供医生远程监测和开出口服窄谱抗生素,以实现更有效的治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acbb/10231770/24bddf7881ed/pone.0285881.g001.jpg

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