Department of Human Anatomy, Wannan Medical College, 241002 Wuhu, Anhui, China.
Department of Medical Technology, Qingdao Binhai University, 266555 Qingdao, Shandong, China.
J Integr Neurosci. 2023 May 8;22(3):63. doi: 10.31083/j.jin2203063.
To investigate the potential mechanisms underlying the migration of endogenous neural stem cells (eNSCs) to the frontal cortex to differentiate into neurons, and to monitor the effect of electroacupuncture (EA) regulation of focal cerebral ischemia (FCI) in rats on the expression of growth arrest-specific protein 7 (Gas7) and nerve growth factor (NGF) in the prefrontal cortex (PFC).
Randomly, forty-eight male Sprague-Dawley rats were divided into four groups: Normal, Sham operation, Model, and EA. The right middle cerebral artery was embolized utilizing the thread-embolism technique. In the EA group, "Baihui" and "Zusanli" points were treated with electroacupuncture for 30 minutes, once a day, for 21 days. Nissl staining revealed the neuronal morphology of the PFC. Using immunohistochemistry and Western blot, the expression of Gas7 and NGF in the right PFC was observed.
Nissl staining showed clear PFC neurons with centered nuclei and distinct nucleoli in the Normal and Sham groups. In the Model group, the PFC nuclei were distinctively smaller. The neuronal morphology in the EA group resembled that of the Normal group. Results from Western blot and immunohistochemistry were comparable. The expression of Gas7 and NGF in the Sham surgery group did not differ significantly from the Normal group. However, the expression of Gas7 and NGF in the Model group was significantly lower than in the Normal group. The expression of Gas7 and NGF was significantly higher in the EA group than in the Model group.
EA can increase the expressions of Gas7 and NGF in the ischemic prefrontal cortex, which may be one of the mechanisms by which EA promotes the differentiation of eNSCs into neurons in the injured area.
探讨内源性神经干细胞（eNSC）迁移到前额皮质分化为神经元的潜在机制，并观察电针对大鼠局灶性脑缺血（FCI）的调节作用对前额皮质（PFC）中生长停滞特异性蛋白 7（Gas7）和神经生长因子（NGF）表达的影响。
将 48 只雄性 Sprague-Dawley 大鼠随机分为四组：正常组、假手术组、模型组和电针组。采用线栓法栓塞右侧大脑中动脉。电针组每天针刺“百会”和“足三里”穴 30 分钟，共 21 天。尼氏染色显示 PFC 的神经元形态。采用免疫组织化学和 Western blot 观察右侧 PFC 中 Gas7 和 NGF 的表达。
尼氏染色显示正常组和假手术组的 PFC 神经元细胞核居中，核仁明显。模型组的 PFC 细胞核明显较小。电针组的神经元形态类似于正常组。Western blot 和免疫组化结果相似。假手术组的 Gas7 和 NGF 表达与正常组无显著差异。然而，模型组的 Gas7 和 NGF 表达明显低于正常组。电针组的 Gas7 和 NGF 表达明显高于模型组。
电针可以增加缺血性前额皮质中 Gas7 和 NGF 的表达，这可能是电针促进损伤区 eNSC 分化为神经元的机制之一。
