Department of Human Anatomy, School of Basic Medical Sciences, Wannan Medical College.
Department of Ultrasound Medicine, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui.
Neuroreport. 2024 Jul 1;35(10):664-672. doi: 10.1097/WNR.0000000000002050. Epub 2024 May 16.
Traditional Chinese medicine (TCM) has long been used to treat various diseases, including cerebral ischemia. The specific molecular mechanism of TCM in the treatment of cerebral ischemia, however, is still unclear. This study investigated the effects of gastrodin, electroacupuncture and their combination on cerebral ischemic rats. We used Nissl staining, immunohistochemical staining and immunoblotting to detect the expression changes of brain-derived neurotrophic factor (BDNF) and interleukin-6 (IL-6) in the frontal cortex. The results showed that the combination therapy of gastrodin and electroacupuncture significantly increased the number of Nissl-positive neurons and improved cell morphology compared with other groups. Mechanistically, we found that the combination of gastrodin and electroacupuncture treatment group can restore the abnormal morphology of neuronal cells caused by cerebral ischemia by rebalancing the expression levels of BDNF and IL-6. Our research indicates that gastrodin combined with electroacupuncture has a significant protective effect on cerebral ischemic injury in rats, possibly by regulating the expression of BDNF and IL-6. This combination therapy is superior to single-drug or electroacupuncture therapy.
中药（TCM）长期以来一直被用于治疗各种疾病，包括脑缺血。然而，TCM 治疗脑缺血的具体分子机制尚不清楚。本研究探讨了天麻素、电针对脑缺血大鼠的影响及其联合作用。我们使用尼氏染色、免疫组织化学染色和免疫印迹检测额皮质中脑源性神经营养因子（BDNF）和白细胞介素-6（IL-6）的表达变化。结果表明，与其他组相比，天麻素和电针联合治疗组显著增加了尼氏阳性神经元的数量，并改善了细胞形态。从机制上讲，我们发现天麻素和电针联合治疗组通过重新平衡 BDNF 和 IL-6 的表达水平，可以恢复脑缺血引起的神经元细胞异常形态。我们的研究表明，天麻素联合电针对大鼠脑缺血损伤具有显著的保护作用，可能通过调节 BDNF 和 IL-6 的表达。这种联合治疗优于单一药物或电针治疗。
